甘肃省文县和迭部县利什曼原虫感染犬的空间聚集性分析  

Analysis of spatial clustering of dogs infected with Leishmania in Wenxian and Diebu counties of Gansu Province

作　　者：余大为[1] 李凡[1] 冯宇[1] 杨成明[1] 杨俊克[1] 刘林林 张永福 Yu Dawei;Li Fan;Feng Yu;Yang Chengming;Yang Junke;Liu Linlin;Zhang Yongfu(Parasitic Disease Prevention and Control Department,Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730020,China;Wenxian Center for Disease Control and Prevention,Wenxian 746400,China;Diebu Center for Disease Control and Prevention,Diebu 747400,China)

机构地区：[1]甘肃省疾病预防控制中心寄生虫病防制科,兰州730020 [2]甘肃省文县疾病预防控制中心,746400 [3]甘肃省迭部县疾病预防控制中心,747400

出　　处：《中华地方病学杂志》2021年第6期431-434,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81703171)。

摘　　要：目的了解甘肃省文县和迭部县利什曼原虫感染犬的空间分布特征,为实施精准防控措施提供科学依据。方法2019年,采用整群抽样方法,在甘肃省内脏利什曼病流行区文县兴隆村和迭部县洛大村采集村内所有犬的血样,选择内脏利什曼病诊断引物RV1-RV2对犬血DNA进行PCR扩增,检测犬利什曼原虫感染情况;并采用SaTScan V9.5软件对犬利什曼原虫感染情况进行空间聚集性分析。结果共调查犬537只,感染阳性犬221只,阳性率为41.2%;其中,文县和迭部县犬利什曼原虫感染阳性率分别为64.6%(95/147)、32.3%(126/390)。SaTScan分析结果显示,文县和迭部县利什曼原虫感染犬在空间范围内聚集性均无统计学意义(P均>0.05)。结论文县和迭部县利什曼原虫感染犬无空间聚集性,但犬利什曼原虫感染阳性率较高,存在较高流行风险。建议当地加强健康教育工作,严格驱蛉灭蛉、查杀病犬,降低内脏利什曼病传播风险。Objective To analyze the spatial distribution characteristics of dogs infected with Leishmania in Wenxian and Diebu counties of Gansu Province,and provide a scientific basis for implementation of precise prevention and control measures.Methods In 2019,blood samples were collected from all dogs in visceral leishmaniasis endemic areas Xinglong Village,Wenxian County and Luoda Village,Diebu County,Gansu Province,by cluster sampling method.The primer RV1-RV2 which was commonly used to diagnose visceral leishmaniasis,was used for PCR amplification of dog blood samples.SaTScan V9.5 software was used to analyze the spatial clustering of dogs infected with Leishmania.Results A total of 537 dogs were investigated,and the number of positive infection was 221,with a positive rate of 41.2%.Among them,the positive rates of dogs infected with Leishmania in Wenxian and Diebu counties were 64.6%(95/147)and 32.3%(126/390),respectively.The results of SaTScan analysis showed that the spatial clustering of dogs infected with Leishmania in Wenxian and Diebu counties were not statistically significant(P>0.05).Conclusions There are no spatial clustering of dogs infected with Leishmania in Wenxian and Diebu counties,but the positive rate of dogs infected with Leishmania is higher,and there is a higher risk of epidemic.It is recommended to strengthen health education in local area,strictly drive out lacewings,detect and kill diseased dogs,and reduce the risk of visceral leishmaniasis transmission.

关 键 词：利什曼原虫属 犬科 空间分布 

分 类 号：S858.292[农业科学—临床兽医学] R531.6[农业科学—兽医学]