基于网络药理学方法的加味黄芪赤风汤治疗IgA肾病作用机制的研究  

Study on the action mechanism of modified Huangqi Chifeng Decoction in the treatment of IgA nephropathy based on network pharmacology method

作　　者：马思佳 赵明明[1] 常美莹 余怡 王如梦 张昱[1] MA Sijia;ZHAO Mingming;CHANG Meiying;YU Yi;WANG Rumeng;ZHANG Yu(Department of Nephrology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院肾病科,北京100091

出　　处：《中国医药导报》2021年第17期29-35,F0004,共8页China Medical Herald

基　　金：首都卫生发展科研专项项目(首发2018-2-4173);首都临床诊疗技术研究及示范应用项目(Z191100006619063)。

摘　　要：目的利用网络药理学知识探讨加味黄芪赤风汤治疗IgA肾病的作用靶点及潜在作用机制。方法在中药系统药理学数据库与分析平台筛选出加味黄芪赤风汤中7味中药相关的化学成分及其作用靶点,依据相应标准分别筛选出中药的活性成分;通过人类孟德尔遗传数据库、GeneCards数据库筛出与IgA肾病相关的疾病靶标,利用R语言计算得到加味黄芪赤风汤与疾病的共同靶标,在Cytoscape研究软件中绘制构建相应的调控网络。采用String数据库构建出对应的蛋白质-蛋白质相互作用网络。运用R语言对靶标进行GO功能和KEGG通路富集分析,确认可能的关键靶点和关键通路。结果在加味黄芪赤风汤中,共筛选出45个活性成分和95个潜在作用靶点。该方治疗IgA肾病可能与影响白蛋白、白细胞介素(IL)-6、丝氨酸/苏氨酸激酶1、血管内皮生长因子A等基因表达有关。GO生物功能分析共包含115条富集结果,主要涉及磷酸酶结合、血红素结合、细胞因子受体结合等过程;KEGG富集得到151条代谢通路,主要包括AGE-RAGE信号通路、IL-17信号通路、肿瘤坏死因子信号通路等。结论通过网络药理学的方法推测加味黄芪赤风汤可通过多方面干预治疗IgA肾病,具有多成分、多靶点、多途径的治疗优势,为日后研究其药效物质基础和作用机制提供了理论参考。Objective To explore the action target and potential mechanism of modified Huangqi Chifeng Decoction in treating IgA nephropathy by using network pharmacology knowledge.Methods Related chemical components of seven traditional Chinese medicine in modified Huangqi Chifeng Decoction and their action targets were screened out from the traditional Chinese medicine systems pharmacology database and analysis platform,and the active components of traditional Chinese medicine were screened out according to the corresponding standards.The disease targets related to IgA nephropathy were screened out through online Mendelian inheritance in man and Genecards database,and the common targets of modified Huangqi Chifeng Decoction and disease were calculated by R language.The corresponding regulatory network was plotted and constructed in Cytoscape research software.The String database was used to construct the corresponding protein-protein interaction network.The GO function and KEGG pathway enrichment analysis of the target were performed by R language to identify the possible key targets and key pathways.Results A total of 45 active components and 95 potential action targets were screened out from the modified Huangqi Chifeng Decoction.The treatment of IgA nephropathy may be related to the influence of the expression of albumin,interleukin(IL)-6,serine/threonine kinase 1,vascular endothelial growth factor A,and other genes.A total of 115 enrichment results were included in GO biofunction analysis,mainly involving phosphatase binding,heme binding,cytokine receptor binding and other processes.A total of 151 metabolic pathways were obtained by KEGG enrichment,mainly including AGE-RAGE signaling pathway,IL-17 signaling pathway,tumor necrosis factor signaling pathway,etc.Conclusion Based on the network pharmacology method,it is speculated that the modified Huangqi Chifeng Decoction can be used in the treatment of IgA nephropathy through various interventions,and has the therapeutic advantages of multiple components,multiple targets

关 键 词：网络药理学 加味黄芪赤风汤 IGA肾病 作用机制 

分 类 号：R256.5[医药卫生—中医内科学]