基于网络药理学探讨黄芪治疗支气管哮喘的作用机制  

Exploring mechanism of Astragali Radix in the treatment of bronchial asthma on network pharmacology

作　　者：李竹英[1] 陈璐 袁星星 王婷 张玉 连梦尧 李星[1] LI Zhuying;CHEN Lu;YUAN Xingxing;WANG Ting;ZHANG Yu;LIAN Mengyao;LI Xing(Department of Respiratory,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin 150040,China;Graduate School,Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin 150040,China;Institute of Pathology,Qiqihar Medical University,Heilongjiang Province,Qiqihar 161000,China)

机构地区：[1]黑龙江中医药大学附属第一医院呼吸科,黑龙江哈尔滨150040 [2]黑龙江中医药大学研究生院,黑龙江哈尔滨150040 [3]齐齐哈尔医学院病理学院,黑龙江齐齐哈尔161000

出　　处：《中国医药导报》2021年第18期4-8,12,F0003,F0004,共8页China Medical Herald

基　　金：国家自然科学基金面上项目(82074365);黑龙江省自然科学基金面上项目(H2017068);黑龙江省哈尔滨市科学技术局应用技术研究与开发项目(2017RAXXJ053)。

摘　　要：目的采用网络药理学方法探讨黄芪治疗支气管哮喘可能的作用机制。方法基于中药系统药理学数据库与分析平台获取黄芪活性化学成分及其对应靶点,通过Genecards数据库获取支气管哮喘相关靶点,将黄芪靶点与疾病靶点映射得到黄芪作用于支气管哮喘的潜在靶点。在Cytoscape软件中构建黄芪-化合物-靶点-疾病网络,采用STRING在线数据库获取蛋白质-蛋白质相互作用(PPI)数据并导入Cytoscape中构建PPI网络。利用Bioconductor生物信息软件包对作用靶点进行基因本体论(GO)功能富集分析和日本京都基因和基因组百科全书(KEGG)通路富集分析。结果通过筛选获得19个黄芪活性化合物,得到黄芪活性化合物的预测靶点97个。在Genecards数据库中搜索到支气管哮喘相关靶点5973个。黄芪作用于支气管哮喘的潜在靶点81个。GO和KEGG富集分析结果表明,黄芪主要涉及DNA和RNA的转录调控、细胞因子与转录因子的活性及受体结合、抗氧化应激、激素调节、蛋白质结合和酶活性等生物过程,通过调节AGE-RAGE信号通路、TNF信号通路、PI3K-Akt信号通路和p53信号通路等,发挥治疗支气管哮喘的作用。结论黄芪及其活性成分通过多个靶点、多条通路发挥抗炎、抗氧化、免疫调节等作用,对支气管哮喘所引发的炎症反应、气道高反应性及气道重塑发挥治疗作用。Objective To explore the possible mechanism of Astragali Radix in the treatment of bronchial asthma by network pharmacology.Methods The effective chemical ingredients and corresponding targets of Astragali Radix were obtained based on traditional Chinese medicine systems pharmacology database and analysis platform.The targets related to bronchial asthma were obtained through Genecards database,and the potential targets of Astragali Radix acting on bronchial asthma were obtained by mapping Astragali Radix targets and disease targets.Astragali Radix-compound-target-disease network was constructed by Cytoscape software.Proteinprotein interaction(PPI)data were obtained from the STRING online database and imported into Cytoscape to construct PPI network.The gene ontology(GO)function enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of the targets was carried out using the Bioconductor bioinformatics software package.Results Nineteen active compounds of Astragali Radix were screened and obtained.Ninety-seven predicted targets of active compounds of Astragali Radix were obtained.A total of 5973 targets related to bronchial asthma were found in Genecards database.A total of 81 potential targets of Astragali Radix on bronchial asthma were obtained.The results of GO and KEGG enrichment analysis showed that DNA and RNA transcription regulation,activity and receptor binding of cytokines and transcription factors,anti-oxidant stress,hormone regu lation,protein binding,enzyme activity and other biological processes were mainly involved by adjusting AGE-RAGE signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,p53 signaling pathway and other pathways to exert its treatment of bronchial asthma.Conclusion Astragali Radix and its active ingredients exert anti-inflammatory,anti-oxidation,immune regulation and other effects through various targets and multiple pathways,and play a therapeutic role in inflammation,airway hyperresponsiveness and airway remodeling caused by br

关 键 词：黄芪 支气管哮喘 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]