电化学法检测细胞中的尿酸和嘌呤  

Detection of Uric Acid and Purine in Cells by Electrochemical Method

作　　者：周实[1] 李锦莲[1] 郭晓玲[1] 张树萌 孟令仁 武冬梅[1] ZHOU Shi;LI Jinlian;GUO Xiaoling;ZHANG Shumeng;MENG Lingren;WU Dongmei(College of Pharmacy,Jiamusi University,Jiamusi Heilongjiang 154007,China)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《佳木斯大学学报（自然科学版）》2021年第3期116-118,共3页Journal of Jiamusi University：Natural Science Edition

基　　金：黑龙江省省属高等学校基本科研业务费科研项目资助(2017-KYYWF-0591)。

摘　　要：研究氧化石墨烯量子点/多壁碳纳米管/丝网印刷电极(GOQDs/MWCNTs/SPCE*)对V79细胞的最佳检测条件.富集电位为0 V、富集时间为240 s、pH为7.4时尿酸和嘌呤标准品混合溶液的电化学信号最佳.该电极实现了对V79细胞中尿酸、黄/鸟嘌呤、腺嘌呤和次黄嘌呤的检测,为生物样品中生物分子的灵敏快速检测提供新的技术方法.To study the optimal conditions for the detection of V79 cells by graphene oxide quantum dots/multi walled carbon nanotubes/screen printed electrode(GOQDs/MWCNTs/SPCE*).The best electrochemical signal was obtained when the enrichment potential was 0 V,the enrichment time was 240 s and pH was 7.4.The electrode can detect uric acid,xanthine/guanine,adenine and hypoxanthine in V79 cells,which provides a new method for the sensitive and rapid detection of biomolecules in biological samples.

关 键 词：电化学 细胞 尿酸 嘌呤 

分 类 号：O657.1[理学—分析化学]