淫羊藿多糖和绞股蓝多糖对PRRSV弱毒疫苗免疫增强效果的研究  

Study of the immune enhancement effect of Epimedium polysaccharide and Gynostemma polysaccharide on PRRSV attenuated vaccine

作　　者：王甜 郭溢舜 江耀伦 李美娣[1,2] 武力 刘汉清[1] 苏胖 WANG Tian;GUO Yishun;JIANG Yaolun;LI Meidi;WU Li;LIU Hanqing;SU Pang(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Guangdong Huanong Hi-Tech Biological Pharmaceutical Co.,Ltd.,Guangzhou 510642,China;Meizhou Vocational Technical School,Meizhou 514011,China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东华农高科生物药业有限公司,广州510642 [3]广东梅州职业技术学院,广东梅州514011

出　　处：《黑龙江畜牧兽医》2021年第11期122-126,137,共6页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了研究淫羊藿多糖和绞股蓝多糖对PRRSV弱毒疫苗的免疫增强效果,笔者通过体外试验测定了淫羊藿多糖和绞股蓝多糖对Marc-145细胞的安全性,及对PRRSV的抗感染性;通过体内试验采用完全随机法将32头10日龄仔猪平均分成4组,分别为空白对照组、淫羊藿多糖组(免疫前按体重150 mg/kg连续口服3 d,每天1次)、绞股蓝多糖组(免疫前按体重150 mg/kg连续口服3 d,每天1次)和疫苗对照组,除空白对照组外,其余各组在14日龄免疫PRRSV弱毒疫苗,测定免疫前和免疫后7,14,21,28,35天的血清抗体水平、γ干扰素(IFN-γ)和白细胞介素12(IL-12)含量,测定免疫后28,35天血清中和抗体效价。结果表明:淫羊藿多糖和绞股蓝多糖对Marc-145细胞最大安全浓度分别为62.5μg/mL和125μg/mL;淫羊藿多糖和绞股蓝多糖对PRRSV的抗感染浓度分别为31.25μg/mL和62.5μg/mL。淫羊藿多糖组在免疫后14,21,28,35天血清抗体水平显著高于疫苗对照组(P<0.05),在免疫后28,35天IFN-γ、IL-12含量显著高于疫苗对照组(P<0.05);绞股蓝多糖组免疫后28,35天血清抗体水平和IFN-γ含量显著高于疫苗对照组(P<0.05),免疫后21,28,35天IL-12含量显著高于疫苗对照组(P<0.05)。淫羊藿多糖组与绞胶蓝多糖组的血清中和抗体效价与疫苗对照组差异不显著(P>0.05)。说明淫羊藿多糖、绞股蓝多糖均对PRRSV弱毒疫苗具有良好的免疫增强作用。In order to study the immune enhancement effect of Epimedium polysaccharide and Gynostemma polysaccharide on PRRSV attenuated vaccine, in vitro experiments were conducted to determine the safety of Epimedium polysaccharide and Gynostemma polysaccharide on Marc-145 cells, as well as their resistance to PRRSV infection. In the in vivo test, 32 10-day-old piglets were divided into 4 groups by a completely random method, namely the blank control group, the Epimedium polysaccharide group(before immunization by oral administration of 150 mg/kg body weight for 3 consecutive days, once a day),and the Gynostemma polysaccharide group(before immunization by oral administration of 150 mg/kg body weight for 3 consecutive days, once a day),and the vaccine control group. Except for the blank control group, the other groups were immunized with the PRRSV attenuated vaccine at 14 days of age, and the serum antibody levels, IFN-γ and IL-12 levels before immunization and 7,14,21,28,and 35 days after immunization were measured, and the serum neutralizing antibody titers were determined on 28 and 35 days after immunization. The results showed that the maximum safe concentrations of Epimedium polysaccharide and Gynostemma polysaccharide on Marc-145 cells were 62.5 μg/mL and 125 μg/mL,respectively;the anti-infective concentrations of Epimedium polysaccharide and Gynostemma polysaccharide against PRRSV were 31.25 μg/mL and 62.5 μg/mL,respectively. The Epimedium polysaccharide group had significantly higher serum antibody levels than the vaccine control group on 14,21,28,and 35 days after immunization(P<0.05);on 28,35 days after immunization, the levels of IFN-γ,IL-12 and were significantly higher than that of the vaccine control group(P<0.05). On 28,and 35 days after immunization in the Gynostemma polysaccharide group, the serum antibody level and the IFN-γ content were significantly higher than that of the vaccine control group(P<0.05),on 21,28,and 35 days after immunization, the IL-12 content was significantly higher than that o

关 键 词：中药多糖 PRRSV弱毒疫苗 淫羊藿 绞股蓝 免疫增强 仔猪 

分 类 号：S852.659.6[农业科学—基础兽医学] S852.43[农业科学—兽医学]