磁珠负载的适配体修饰纳米金探针用于血清中细菌内毒素检测  

Magnetic Beads-Loaded Aptamer-Modified Gold Nano Probes for Detection of Bacterial Endotoxin in Serum

作　　者：方凯 郜毓堃 杨丽敏[1] 姜磊[1] Fang Kai;Gao Yukun;Yang Limin;Jiang Lei(State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology,China University of Petroleum(East China),Qingdao,266580)

机构地区：[1]中国石油大学(华东)重质油国家重点实验室及生物工程与技术中心,青岛266580

出　　处：《化学通报》2021年第6期563-570,513,共9页Chemistry

基　　金：国家自然科学基金项目(31871878)资助。

摘　　要：生物传感检测血清中的细菌内毒素具有重要的应用价值,但其开发过程受到血清所含物质复杂性的限制。本文开发了一种基于磁珠-纳米金-适配体(MB-AuNPs-APT)的生物探针比色传感器。通过生物探针捕获内毒素来影响其过氧化物酶活性,进而影响H2O2催化3,3′,5,5′-四甲基联苯胺(TMB)产生氧化TMB的量,最终达到比色分析内毒素的目的。分析了该方法对血清中内毒素检测的可行性,结果表明,基于MB-AuNPs-APT生物探针比色传感法的检测限为0.402ng/mL,线性范围为0.1~100 ng/mL。在50%血清样品中此生物传感器检测内毒素的回收率为99.59%~112.00%,展示了该生物探针在血清中检测内毒素的可靠性。Bio-sensing detection of bacterial endotoxins(LPS)in serums is of great application value,but its development process is limited by the complexity of the substances contained in the serum.In this paper,a colorimetric sensor based on the magnetic bead-gold nanoparticles-aptamer(MB-AuNPs-APT)bioprobe was developed.LPS is captured by bioprobes to affect its peroxidase activity,this in turn affects the production which H2O2 catalytic TMB producing oxTMB,and finally achieve the purpose of colorimetric analysis of LPS.Meanwhile,the feasibility of LPS detection in serum is also analyzed.The results show that the detection limit using the colorimetric sensing method based on MB-AuNPs-APT bioprobes is 0.402 ng/mL and the linear range is 0.1~100 ng/mL.In the detection of LPS in 50%serum,the recovery rate of this biosensor to detect LPS ranged from 99.59 to 112.00%,demonstrated the reliability of the biological probe detecting LPS in serum.

关 键 词：生物传感 内毒素 纳米金 适配体 血清 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]