Characterization of sensory neuron membrane proteins(SNMPs)in cotton bollworm Helicoverpa armigera(Lepidoptera:Noctuidae)  

作　　者：Wei Xu Huijie Zhang Yalin Liao Alexie Papanicolaou 

机构地区：[1]Department of Agricultural Sciences,Murdoch University,Murdoch,Australia [2]Key Laboratory of Biochemistry and Molecular Pharma-cology of Chongqing,Chongqing Medical University,Chongqing,China [3]State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing,China [4]Hawkesbury Institute for the Environment,Western Sydney University,Richmond,Australia

出　　处：《Insect Science》2021年第3期769-779,共11页昆虫科学（英文版）

基　　金：We would like to thank Helicoverpa genome consortium for genome sequences and transcripome data.We also thank Professor Myron Zalucki of University of Queensland providing us H.armigera pupae.Dr.Wei Xu is the recipient of an Australian Research Council Discovery Early Career Researcher Award(DECRA)(DEI 60100382).

摘　　要：Sensory neuron membrane proteins(SNMPs)play a critical role in insect chemosensory system.Previously,three SNMPs were identified,characterized and functionally investigated in a lepidopteran model insect,Bombyx mori.However,whether these results are consistent across other lepidopteran species are unknown.Here genome and transcriptome data analysis,expression profiling,quantitative real-time PCR(qRT-PCR)and the yeast hybridization system were utilized to examine snmp genes of Helicoverpa armigera,one of the most destructive lepidopteran pests in cropping areas.In silico expression and qRT-PCR analyses showed that,just as the B.mori snmp genes,H.armigera snmpl(Harmsnmpl)is specifically expressed in adult antennae.Harmsnmp2 is broadly expressed in multiple tissues including adult antennae,tarsi,larval antennae and mouth-parts.Harmsnmp3 is specifically expressed in larval midguts.Further RNAseq analysis suggested that the expression levels of Harmsnmp2 and Harmsnmp3 differed significantly depending on the plant species on which the larvae fed,indicating they may be involved in plant-feeding behaviours.Yeast hybridization results revealed a protein-protein interaction between HarmSNMPl and the sex pheromone receptor,HarmOR13.This study demonstrated that SNMPs may share same functions and mechanisms in different lepidopteran species,which improved our understanding of insect snmp genes and their functions in lepidopterans.

关 键 词：expression profile insect olfactory system insect-plant interaction QRT-PCR 

分 类 号：S435[农业科学—农业昆虫与害虫防治]