基因重组技术在猪伪狂犬病毒基因工程疫苗中的研究进展  

Research Progress of Gene Recombination Technology in Genetic Engineering Vaccine of Porcine Pseudorabies Virus

作　　者：柯海意 王帅 徐民生 蔡汝健 勾红潮 臧莹安 李春玲 简运华 KE Haiyi;WANG Shuai;XU Minsheng;CAI Rujian;GOU Hongchao;ZANG Ying’an;LI Chunling;JIAN Yunhua(Zhongkai University of Agricultural Engineering,Guangzhou 510305,China;Institute of Animal Health,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Animal and Poultry Epidemic Prevention and Control/Guangdong Science Observation and Laboratory Station of Veterinary Drugs and Diagnostic Technology,Ministry of Agriculture and Rural Affairs,Guangzhou 510640,China;Maoming Animal Epidemic Control Center,Maoming 525000,China;Guangdong Guangken Animal Husbandry Group Co.,Ltd.Guangzhou 510612,China)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510305 [2]广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业农村部兽用药物与诊断技术广东科学观测实验站,广东广州510640 [3]茂名市动物疫病控制中心,广东茂名525000 [4]广东广垦畜牧集团股份有限公司,广东广州510612

出　　处：《广东农业科学》2021年第5期124-131,共8页Guangdong Agricultural Sciences

摘　　要：猪伪狂犬病是一种由伪狂犬病病毒(Pseudorabies virus,PRV)感染引起的急性高度接触性传染病,可造成不同日龄的猪发病,给养猪业造成了严重的经济损失。预防、控制甚至消灭猪伪狂犬病的主要措施之一是疫苗免疫接种。通过基因重组技术对PRV关键毒力基因进行改造,敲除毒力基因或插入免疫增强基因,是降低PRV毒力或提高病毒免疫保护效果的有效方法之一,也是研制安全高效PRV基因工程疫苗的重要手段。从基因同源重组、Cre/lox P位点特异性重组、细菌人工染色体和CRISPR基因编辑等不同基因重组技术应用方面对猪PRV基因工程疫苗的研制进行综述,为PRV重组疫苗的进一步深入研究提供参考。Porcine pseudorabies is an acute and highly contagious disease caused by pseudorabies virus(PRV)infection,which can cause disease in pigs of different ages and serious economic losses to the pig industry.One of the main measures to prevent,control and even eradicate porcine pseudorabies is vaccination.It is one of the effective methods to reduce the virulence of PRV or improve the immune protection effect of virus by transforming the key virulence genes of PRV,knocking out the virulence genes or inserting the immune enhancement genes through gene recombination technology,and it is also an important means to develop a safe and efficient genetic engineering vaccine of PRV.The development of porcine PRV genetic engineering vaccine was reviewed from the application of different gene recombination technologies,such as homologous recombination,Cre/loxP site-specific recombination,bacterial artificial chromosome and CRISPR gene editing,with a view to provide references for further study of PRV recombinant vaccine.

关 键 词：猪伪狂犬病毒 基因工程疫苗 基因编辑 同源重组 位点特异 CRISPR 

分 类 号：S852.659.1[农业科学—基础兽医学]