吉林省鼠伤寒沙门菌MLVA分型流行特征分析  

Epidemic Characteristics of MLVA typing of Salmonella typhimurium isolates in Jilin Province

作　　者：王岙[1] 赵薇[1] 李可维[1] 郭丰 石奔 杨修军[1] 孙景昱 WANG Ao;ZHAO Wei;LI Ke-wei;无(Jilin Provincial Center for Disease Control and Prevention·Jilin Provincial Institute of Public Health,Changchun 130062,China;Jilin University School of Public Health,Changchun 130021,China)

机构地区：[1]吉林省疾病预防控制中心·吉林省公共卫生研究院,吉林长春130062 [2]吉林大学公共卫生学院,吉林长春130021

出　　处：《中国实验诊断学》2021年第5期737-740,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生科技能力提升计划(2020Z016,2014Q012)。

摘　　要：目的探讨吉林省鼠伤寒沙门菌多位点可变数目串联重复序列(MLVA)分型的流行特征,为食源性腹泻感染的预防控制提供依据。方法根据国际Pulse Net公布的鼠伤寒沙门菌MLVA分型方法对吉林省2014-2016年间食源性感染腹泻病例分离的71株鼠伤寒沙门菌进行流行特征分析。结果71株菌中46株分离自男性,25株分离自女性;以0-8岁儿童居多(59.2%);夏季为流行高峰期。聚类分析显示,71株菌被分为5大基因群(A-E群)37个基因型,B基因群(78.9%)为吉林省近年主要的流行菌群。吉林省6个地区(长春、吉林、延边、白城、辽源、通化)主要流行B群,通化市亦流行E群,辽源市亦流行A群,通化市、吉林市、辽源市的基因型分布稍多于其他地区。结论吉林省流行的鼠伤寒沙门菌存在着显著的遗传多态性,且同一克隆菌株在相同地区内流行,亦在不同地区间交叉流行。Objective To explore the epidemic characteristics of multiple-locus variable number tandem repeat analysis(MLVA)typing of Salmonella typhimurium in Jilin Province,and to provide a basis for the prevention and control of foodborne diarrhea infection.Methods According to the MLVA typing method of Salmonella typhimurium published by Pulse Net International network,the epidemic characteristics of 71strains of Salmonella typhimurium isolated from food-borne diarrhea cases in Jilin Province from 2014to 2016were analyzed.Results Among the 71strains,46strains were isolated from men and 25strains were isolated from women;children aged 0-8were the majority(59.2%);the third quarter was the peak of the epidemic.Cluster analysis showed that the 71strains were divided into 5genotypes(A-E)and 37genotypes,and the B genotypes(78.9%)were the main epidemic flora in Jilin province in recent years.Group B was mainly prevalent in six regions of Jilin Province(Changchun,Jilin,Yanbian,Baicheng,Liaoyuan,and Tonghua).The E group was also prevalent in Tonghua city,and the A group was also prevalent in Liaoyuan city.The genotype distribution of Tonghua City,Jilin City and Liaoyuan city was slightly higher than that of other regions.Conclusion The salmonella typhimurium prevalent in Jilin province from 2014to 2016showed obvious genetic polymorphism,and the same clone was prevalent in the same region,but also cross-prevalent in different regions.

关 键 词：鼠伤寒沙门菌 MLVA 基因分型 吉林省 

分 类 号：R378.2[医药卫生—病原生物学]