分子标记辅助选育甘蓝型油菜高油酸pol TCMS不育两用系及其恢复系  

Breeding for thermo-sensitive pol cytoplasmic male sterile line and its restorer with high oleic acid through molecular marker-assisted selection in Brassica napus

作　　者：郭聚领 石笑蕊 辛强[1] 洪登峰[1] 杨光圣[1] GUO Ju-ling;SHI Xiao-rui;XIN Qiang;HONG Deng-feng;YANG Guang-sheng(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070

出　　处：《中国油料作物学报》2021年第3期418-425,共8页Chinese Journal of Oil Crop Sciences

基　　金：华中农业大学校资助创新(2662017PY02);作物遗传改良国家重点实验室开放课题(ZK201909)。

摘　　要：高油酸油菜的食用品质、营养价值、贮存和加工品质较高,在食用、工业和药用等领域都发挥重要的作用。为获得高油酸油菜不育系和恢复系,以高油酸油菜J-3111为供体亲本,采用回交转育与分子标记辅助选择相结合的育种策略,将高油酸优良等位基因(fad2基因)导入到甘蓝型油菜波里马细胞质温敏雄性不育(pol TCMS)两用系616A及其恢复系L-135R中。结果表明:改良株系油酸含量最高可达75.18%;同时,改良株系在角果长度、每角果粒数和千粒重等产量性状与轮回亲本无显著差异。此外,利用重测序数据开发了油酸含量调控位点连锁的In‐Del标记。由此本研究获得了甘蓝型油菜高油酸pol TCMS不育两用系及其恢复系,为培育高产高油酸油菜新品种奠定了基础。High oleic rapeseed(Brassica napus L.)oil has high nutrition with good quality for cooking,processing and storage.For high oleic acid breeding and heterosis utilization,male sterile line and restoring line were bred by using line J-3111 with high oleic acid as donor parent.Its high oleic allele(fad2 gene)was succefully transferred into a thermo-sensitive pol cytoplasmic male sterile(pol TCMS)line 616A and a restoring line L-135R by backcross and molecular marker-assisted selection.Results showed that oleic content of the improved strains was up to 75.18%.Meanwhile,yield traits of the improved lines showed no significant difference comparing with recipient parents in silique length,seeds number per silique and thousand-seeds weight.Based on whole-genome resequencing data,InDel markers were developed closely-linked with fad2 gene.Thus we obtained improved pol TCMS line and restoring line with high oleic contents,which laid foundation for breeding of high-yield and high-oleic cotent.

关 键 词：甘蓝型油菜 回交育种 分子标记辅助选择 细胞质温敏雄性不育两用系 高油酸 

分 类 号：S565.4[农业科学—作物学]