miR-5189-3p在下腔深静脉血栓形成的调控机制的研究  

Study on the regulatory mechanism of miR-5189-3p in deep venous thrombosis of lower extremities

作　　者：冯涛[1] 李晶[2] 潘金强[1] 郑殿宇 鲁静[3] 耿中利[1] 马震[1] FENG Tao;LI Jing;PAN Jinqiang(Department of General Surgery,Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Xinjiang,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院普外二科,乌鲁木齐830000 [2]新疆医科大学附属中医医院内分泌科,乌鲁木齐830000 [3]新疆维吾尔自治区人民医院妇科

出　　处：《临床外科杂志》2021年第5期478-481,共4页Journal of Clinical Surgery

摘　　要：目的通过动物模型实验探讨miR-5189-3p在下肢深静脉血栓形成中的表达调控机制。方法选取48只SPF级SD大鼠,随机分为4组,正常对照组(A组)、单纯下腔静脉血栓模型组(B组)、hsa-miR-5189-3p模拟物(hsa-miR-5189-3p mimics)组(C组)和hsa-miR-5189-3p模拟物阴性对照(hsa-miR-5189-3p NC)组(D组),每组12只。进行SD大鼠DVT建模,建模完成24小时后,对4组SD大鼠进行颈椎脱臼法处死,进行下腔静脉血栓SD大鼠成栓情况的观察及组织取材;使用病理学HE染色法切片观察SD大鼠下腔静脉及内容物组织病理改变;实时荧光定量PCR技术检测SD大鼠下腔静脉组织JAG1、Notch1和Hes1的表达差异。结果 miRNA模拟物组和miRNA模拟物阴性对照组,血栓组织中的有核细胞较多,机化程度增加,血栓外周区域有部分小裂隙产生和形成,从而形成管腔样结构,有血流再通的征象。JAG 1、Notch 1、Hes 1的实时荧光定量PCR结果显示:上调miR-5189-3p的表达量后,JAG1、Notch1和Hes1的表达量明显上升(P<0.05)。结论 hsa-miR-5189-3p可能为下肢深静脉血栓形成的关键miRNA。Objective To investigate the expression regulation mechanism of miR-5189-3 p in deep venous thrombosis of lower limbs through animal model test.Methods 48 SPF SD rats were selected.They were randomly divided into 4 groups: normal control group(group A),simple inferior vena cava thrombosis model group(group B),hsa-miR-5189-3 p mimics group(group C) and hsa-miR-5189-3 p NC group(group D),with 12 mice in each group.DVT modeling was carried out in SD rats.24 hours after the modeling was completed, four groups of SD rats were sacrificed for cervical dislocation, and the thromboembolization of SD rats with inferior vena cava thrombosis was observed and tissue samples were collected.Pathological changes of SD rats’ inferior vena cava and its contents were observed by pathological HE staining sections.Real-time fluorescence quantitative PCR was used to detect the expression differences of JAG1,Notch1 and Hes1 in inferior vena cava tissues of SD rats.Results In the miRNA mimics group and the miRNA mimics negative control group, there were more nucleated cells in the thrombotic tissue, the degree of organization increased, and some small fissures were generated and formed in the peripheral area of thromboembolism, thus forming the lumen like structure with signs of blood flow recanalization.Real-time fluorescence quantitative PCR results of JAG1,Notch1 and Hes1 showed that after upregulation of miR-5189-3 p, the expression levels of JAG1,Notch1 and Hes1 increased significantly(P<0.05).Conclusion hsa-miR-5189-3 p may be a key miRNA for deep venous thrombosis of lower extremities.

关 键 词：静脉血栓 静脉内皮细胞 NOTCH信号通路 

分 类 号：R543.6[医药卫生—心血管疾病]