基于转录组测序的湖北海棠抗白粉病机制分析  

Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis

作　　者：兰黎明 罗昌国[2] 王三红[1] LAN Liming;LUO Changguo;WANG Sanhong(Laboratory of Fruit Tree Biology,College of Horticulture,Nanjing Agricultural University,Nanjing 210018,China;Institute of Fruits Sciences f Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]南京农业大学园艺学院,果树生物技术实验室,南京210018 [2]贵州省农业科学院果树科学研究所,贵阳550006

出　　处：《园艺学报》2021年第5期860-872,共13页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31171935,31560551)。

摘　　要：为研究湖北海棠[Malus hupehensis(Pamp)Rehd.]响应白粉病菌胁迫过程中相关基因的表达及分子机制,取同一时期爆发白粉病的‘青砧1号’、‘青砧1号’/湖北海棠嫁接苗的接穗部分和湖北海棠等3份材料的叶片,使用BGISEQ-500测序技术对其进行转录组测序分析。结果表明:青砧1号、青砧1号/湖北海棠和湖北海棠对白粉病的抗性依次增加;GO富集显示代谢过程和催化活性相关的GO term在青砧1号、青砧1号/湖北海棠和湖北海棠中被显著富集;KEGG分析显示亚油酸代谢、植物病原体相互作用、磷酸肌醇代谢、油菜素类固醇生物合成和磷脂酰肌醇信号系统等5条代谢途径在‘青砧1号’/湖北海棠和湖北海棠中均显著富集,碳代谢途径只在抗性强的湖北海棠中显著富集。与白粉病抗性相关基因家族表达分析显示,WRKY70、WRKY33、NPR3和WRKY40等转录因子基因在青砧1号/湖北海棠和湖北海棠中较青砧1号显著上调表达。实时荧光定量PCR结果证实了转录组测序结果的准确性。To explore the pathways and mechanism of response to powdery mildew in Malus hupehensis(Pamp.)Rehder,we used the BGISEQ-500 sequencing technology and de novo transcriptome assembly to gain a comprehensive overview of the transcriptome of the leaves of’Qingzhen 1’, ’Qingzhen 1’/Malus hupehensis and M. hupehensis after the outbreak of powdery mildew in the same period. The resistance to powdery mildew of three materials increased in turn. Gene Ontology(GO)enrichment showed significant enrichment of GO term associated with metabolic process and catalytic activity in Qingzhen 1,Qingzhen 1/Malus hupehensis and M. hupehensis. KEGG analysis showed linoleic acid metabolism,plant-pathogen interaction,inositol phosphate metabolism,brassinosteroid biosynthesis and phosphatidylinositol signaling system etc. were significantly enriched in Qingzhen 1/Malus hupehensis and resistance to powdery mildew,respectively. And the carbon metabolism pathway was only significantly enriched in M. hupehensis. Expression analysis of genes related to the resistance to powdery mildew showed that WRKY70,WRKY33,NPR3 and WRKY40 were significantly up-regulated in Qingzhen 1/Malus hupehensis and M. hupehensis compared to Qingzhen 1. The accuracy of sequencing were confirmed by qRT-PCR experiment.

关 键 词：苹果 湖北海棠 砧木 砧穗互作 白粉病 转录组 差异表达 

分 类 号：S661.4[农业科学—果树学]