田间二化螟雄蛾对不同配比性信息素的嗅觉反应及性信息素识别相关基因表达水平差异  

Variation in the olfactory response and expression levels of genes involved in sex pheromone recognition in male moths of field Chilo suppressalis(Lepidoptera:Crambidae)to sex pheromone blends at different ratios

作　　者：刘天伟[1] 陈运康 许春梅 郭荣[4] 冯波[5] 杜永均[1] LIU Tian-Wei;CHEN Yun-Kang;XU Chun-Mei;GUO Rong;FENG Bo;DU Yong-Jun(Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China;Youxian County Station of Plant Protection, Youxian, Hunan 412300, China;Agricultural and Rural Bureau of Qianwei County, Sichuan Province, Qianwei, Sichuan 614400, China;National Extension and Service Center of Agricultural Technology, Beijing 100125, China;Institute of Health and Environmental Ecology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China)

机构地区：[1]浙江大学农药与环境毒理研究所,杭州310058 [2]湖南攸县植保植检站,湖南攸县412300 [3]犍为县农业农村局,四川犍为614400 [4]全国农业技术推广服务中心,北京100125 [5]温州医科大学健康与环境生态研究所,浙江温州325035

出　　处：《昆虫学报》2021年第5期585-596,共12页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31871956);浙江省重点研发计划(2018C02027)。

摘　　要：【目的】本研究旨在从行为和嗅觉分子水平探究二化螟Chilo suppressalis雄蛾对性信息素嗅觉反应的地理种群差异及其机理,以明确该害虫田间种群雄成虫的嗅觉适应性及其特点。【方法】采用7种Z11-16∶Ald,Z9-16∶Ald和Z13-18∶Ald配比不同的三元性信息素诱芯(Z11-16∶Ald和Z9-16∶Ald配比分别为540μg∶540μg,864μg∶216μg,945μg∶135μg,980μg∶98μg,1003μg∶77μg,1016μg∶64μg和1045μg∶35μg,而Z13-18∶Ald含量保持不变),在中国6省水稻田间诱捕二化螟雄成虫;利用实验室风洞测定864μg∶216μg,980μg∶98μg和1016μg∶64μg配比诱芯在田间诱捕的越冬代和第2代二化螟雄蛾分别对这3个配比的性信息素诱芯的行为反应;并采用RT-qPCR方法测定这7种性信息素配比不同的诱芯诱捕的雄蛾触角中二化螟雄蛾12个性信息素识别相关基因的表达水平。【结果】在田间试验中,Z11-16∶Ald和Z9-16∶Ald不同配比的性信息素诱芯对水稻二化螟雄蛾均有引诱作用,但不同配比引诱的二化螟雄蛾占比不同。6省诱芯中Z11-16∶Ald和Z9-16∶Ald的最佳配比不同,并且同一配比性信息素诱芯在不同省份引诱的二化螟雄蛾占比也不同。结果表明,不仅在不同地区诱芯的性信息素最佳配比不同,而且在不同地区不同配比性信息素的诱蛾量占比也不同。实验室风洞试验中,864μg∶216μg和1016μg∶64μg配比诱芯田间诱捕雄蛾分别对864μg∶216μg和1016μg∶64μg配比诱芯的行为反应均显著强于对其余两种配比诱芯,980μg∶98μg配比诱芯田间诱捕雄蛾对1016μg∶64μg,980μg∶98μg和864μg∶216μg配比诱芯的行为反应无显著差异。在RT-qPCR测定中,除GOBP1外,其余11个性信息素识别相关基因在Z11-16∶Ald和Z9-16∶Ald不同配比诱芯诱捕雄蛾触角中的表达水平均存在显著差异,其中PBP3,PBP4,PR1,PR2,PR4,PR5和PR67个基因的表达水平与性信息素配比之间存在显著的线性相【Aim】This study aims to explore the variation among geographical populations in the olfactory response of male moths of Chilo suppressalis to sex pheromone and the related mechanisms,so as to understand the olfactory adaptation of male adults in field populations of the pest and its characteristics.【Methods】The male moths of C.suppressalis were trapped in rice fields in six provinces of China by using seven lures containing 3-component sex pheromone blends of Z11-16∶Ald and Z9-16∶Ald at different ratios(540μg∶540μg,864μg∶216μg,945μg∶135μg,980μg∶98μg,1003μg∶77μg,1016μg∶64μg and 1045μg∶35μg)and Z13-18∶Ald with the constant content,the behavioral responses of male moths of the overwintering generation and the second generation trapped by the lures containing Z11-16∶Ald and Z9-16∶Ald at the ratios of 864μg∶216μg,980μg∶98μg and 1016μg∶64μg in the filed to the lures at these three ratios were determined by laboratory bioassay in the wind tunnel,and the expression levels of 12 genes involved in sex pheromone recognition in the antennae of male moths trapped by the seven lures containing sex pheromone blends at different ratios were detected by RT-qPCR.【Results】In the field tests,all sex pheromone lures containing different Z11-16∶Ald and Z9-16∶Ald ratios could attract male moths of C.suppressalis,but the proportions of male moths of C.suppressalis trapped by lures containing different Z11-16∶Ald and Z9-16∶Ald ratios were different.The optimal ratio of Z11-16∶Ald and Z9-16∶Ald was different among the six provinces,and the proportion of male moths of C.suppressalis trapped by lure containing the same Z11-16∶Ald and Z9-16∶Ald ratio was different among the six provinces.The results showed that not only the optimal ratios of sex pheromones of lures in different geographical locations were different,the proportions of moths attracted by sex pheromone blends at different ratios in different geographical locations were also different.In the laboratory

关 键 词：二化螟 性信息素 嗅觉 种群差异 诱捕 风洞 

分 类 号：Q968[生物学—昆虫学]