Wood forming tissue-specific expression of PdSuSy and HCHL increases holocellulose content and improves saccharification in Populus  

作　　者：Yang Zhang Hua Xu Yingzhen Kong Jiawen Hua Xianfeng Tang Yamei Zhuang Yue Bai Gongke Zhou Guohua Chai 

机构地区：[1]University of Chinese Academy of Sciences,Beijing 100049,People’s Republic of China [2]Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,People’s Republic of China [3]Shandong Provincial Key Laboratory of Energy Genetics,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,People’s Republic of China [4]College of Agronomy,Qingdao Agricultural University,Qingdao 266109,People’s Republic of China [5]Forestry College,Inner Mongolia Agricultural University,Huhhot 010018,People’s Republic of China [6]College of Resources and Environment,Qingdao Agricultural University,Qingdao 266109,People’s Republic of China

出　　处：《Journal of Forestry Research》2021年第4期1681-1688,共8页林业研究（英文版）

基　　金：funded by National Key Program on Transgenic Research(2018ZX08020002);National Key Scientific Research Project of China(2016YFD0600104);National Natural Science Foundation of China(31670606,31570670,31770315 and 31701068);Major Basic Research Project of Shandong Natural Science Foundation(ZR2018ZC0335);Shandong Provincial Natural Science Foundation(ZR2017BC096 and ZR2017BC078);Taishan Scholar Program of Shandong(to G.Z.)。

摘　　要：Development of strategies to deconstruct lignocellulosic biomass in tree species is essential for biofuels and biomaterials production.We applied a wood forming tissue-specific system in a hybrid poplar to express both PdSuSy(a sucrose synthase gene from Populus deltoides×P.euramericana that has not been functionally characterized)and HCHL(the hydroxycinnamoyl-CoA hydratase-lyase gene from Pseudomonas fluorescens,which inhibits lignin polymerization in Arabidopsis).The PdSuSy-HCHL overexpression poplars correspondingly driven by the promoters of Arabidopsis AtCesA7 and AtC4 H resulted in a significant increase in cellulose(>8%),xylan(>12%)and glucose(>29%)content,accompanying a reduction in galacturonic acid(>36%)content,compared to control plants.The saccharification efficiency of these overexpression poplars was dramatically increased by up to 27%,but total lignin content was unaffected.These transgenic poplars showed inhibited growth characteristics,including>16%reduced plant height,>10% reduced number of internodes,and>18% reduced fresh weight after growth of 4 months,possibly due to relatively low expression of HCHL in secondary xylem.Our results demonstrate the structural complexity and interaction of the cell wall polymers in wood tissue and outline a potential method to increase biomass saccharification in woody species.

关 键 词：SACCHARIFICATION BIOMASS Cell wall composition Growth POPLAR 

分 类 号：S792.11[农业科学—林木遗传育种]