Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome  

作　　者：XIANG Shan XIA Ming-feng SONG Jing-yan LIU Dan-qi LIAN Fang 

机构地区：[1]Gynecology of Traditional Chinese Medicine,the First Clinical College,Shandong University of Traditional Chinese Medicine,Jinan 250355,China [2]Reproductive and Genetic Center of Integrated Traditional and Western Medicine,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,China [3]Experimental Center,Shandong University of Traditional Chinese Medicine,Jinan 250355,China

出　　处：《Chinese Journal of Integrative Medicine》2021年第5期330-335,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Shandong Natural Science Foundation(No.ZR2019QH013);Shandong Natural Science Foundation(No.2018CXGC130);State Administration of Foreign Experts Affairs China(No.P192023031)。

摘　　要：Objective:To evaluate the effect of electro-acupuncture(EA)in infertile patients with phlegmdampness polycystic ovary syndrome-insulin resistance(PCOS-IR).Methods:Seventy-six PCOS-IR patients who underwnet in vitro fertilization and embryo transfer(IVF-ET)were equally assigned to two groups according to a random digital table:the EA group and the control group,with 38 cases in each group.Before undergoing IVF,the two groups were treated with EA or pseudo-acupuncture,respectively,for 3 menstrual cycles.The intervention was 25 min twice a week until the day of oocyte collection.The selected acupoints were Zhongwan(RN 12),Tianshu(ST 25),Daheng(SP 15),Daimai(GB 26),Qihai(CV 6),Guanyuan(CV 4),and bilateral points including Xuehai(SP 10),Fenglong(ST 40),Zusanli(ST 36),and Yinlingquan(SP 9).Evaluation of phlegm-dampness syndrome score and IR score were carried out before and after treatment.Additionally,the number of oocytes retrieved,transplantable embryo rate,high-quality embryo rate,clinical pregnancy rate and live birth rate were compared between the two groups.Real-time polymerase chain reaction analysis was used to monitor the m RNA expression of the insulin receptor substrate(IRS-1),phosphatidylinositiol 3-kinase(PI3 K)and glucose transport factor 4(GLUT4)in ovarian granulosa cells.Results:EA treatment reduced the phlegm-dampness syndrome score as well as the IR scores compared with the control group(P<0.05).No significant differences in the number of oocytes retrieved and clinical pregnancy rate between the two groups(P>0.05).Moreover,the transplantable embryo rate[49.0%(284/580)vs.41.9%(273/652)],high-quality embryo rate[36.6%(104/284)vs.27.8%(76/273)],and live birth rate[50%(19/38)vs.26.3%(10/38)]in the EA group were significantly higher than in the control group(P<0.05).Gene expression analyses revealed significantly elevated IRS-1,PI3 K and GLUT4 m RNA in ovarian granulosa cells of the EA group compared with the control group(P<0.05).Conclusions:EA may ameliorate the effects of phlegm-dampness syndrome and o

关 键 词：polycystic ovary syndrome phlegm-dampness syndrome ELECTRO-ACUPUNCTURE insulin resistance granulosa cells mechanism of action 

分 类 号：R246.3[医药卫生—针灸推拿学]