厌氧砷还原细菌(Clostridium sp.ZY1)分离鉴定及基因组分析  

Isolation,Identification and Genome Analysis of an Anaerobic Arsenic-Reducing Bacterium Clostridium sp. ZY1

作　　者：张堃 周媛 黄华枝 廖俊杰[1] 朱永闯 李湘伟[1] 李林[1] 黄伟忠[1] 房保柱[2] 李文均[2] 李金天 廖斌[2] ZHANG Kun;ZHOU Yuan;HUANG Hua-zhi;LIAO Jun-jie;ZHU Yong-chuang;LI Xiang-wei;LI Lin;HUANG Wei-zhong;FANG Bao-zhu;LI Wen-jun;LI Jin-tian;LIAO Bin(Guangdong Indust.Polytech.,Guangzhou 510300;Schl.of Life Sci.,Sun Yat-Sen Uni.,Guangzhou 510275;Inst.of Eco-Sci.,Schl.of Life Sci.,S.China Normal Uni.,Guangzhou 510631)

机构地区：[1]广东轻工职业技术学院,广东广州510300 [2]中山大学生命科学学院,广东广州510275 [3]华南师范大学生命科学学院生态科学研究所,广东广州510631

出　　处：《微生物学杂志》2021年第2期27-36,共10页Journal of Microbiology

基　　金：广东省自然科学基金项目(2018A0303130014);广东省教育厅特色创新项目(自然科学2018GKTSCX091);广东轻工职业技术学院自然科学项目(KJ2020-003);广东轻工职业技术学院校级专业领军人才项目(KYRC2020-004)。

摘　　要：为了进一步了解砷矿区重金属污染环境中砷还原微生物多样性,利用厌氧富集手段,针对砷矿区底泥样品进行厌氧砷还原功能微生物资源的分离及纯培养,并结合基因组学对分离菌株进行初步分析。经过富集分离获得菌株ZY1,通过16S rRNA基因相似性及系统发育树分析,表明菌株ZY1属于梭菌属(Clostridium),菌株ZY1的最适pH范围为6.4~7.1,NaCl的耐受范围为0%~3%,菌株ZY1能够耐受100 mmol/L五价砷(As(Ⅴ)),三价砷(As(Ⅲ))的耐受浓度仅为1.98 mmol/L。菌株ZY1基因组大小为4.4 Mbp,包含31个Scaffolds, N50为318 327 bp, GC含量为30.6%,预测得到4 210个基因。基因组信息表明,菌株ZY1具有砷还原的相关基因簇,并具有较好的抗逆代谢通路。研究结果可为矿山酸性废水底泥微生物多样性研究提供参考,并为砷污染环境的修复及生态治理研究提供菌种资源。In order to further understand the diversity of arsenic reducing microorganisms in heavy metal polluted environment of arsenic mining area, the anaerobic enrichment method was used to isolate and pure culture the anaerobic arsenic reduction microbial resources from bottom mud in arsenic mining area, and the isolated strains were preliminarily analyzed combined with genomics. 16 S rRNA gene similarity and phylogenetic tree analyses showed that strain ZY1 belonged to the genus Clostridium. The optimal pH range of strain ZY1 was 6.4-7.1, and the tolerance range of NaCl was 0%-3%. Strain ZY1 could tolerate 100 mmol/L As(V), and the tolerance concentration of As(Ⅲ) was only at 1.98 mmol/L. The genome size of strain ZY1 was 4.4 Mbp, including 31 scaffolds, N50 318 327 bp and GC content 30.6%, and predicted obtained 4 210 genes. Genomic information showed that ZY1 had arsenic reduction related gene clusters and had a resistance metabolic pathway. This study has laid theoretical foundation for the study on microbial diversity of acid mine wastewater bottom mud, and the results provided strain seed resources for the remediation and ecological treatment of arsenic polluted environment.

关 键 词：砷矿 砷酸盐还原细菌 基因组 纯培养 梭菌 

分 类 号：Q93-331[生物学—微生物学]