肠道菌群对蛋氨酸脑啡肽治疗肿瘤疗效的影响  

Influence of Intestinal Microbial Population on Therapeutic Effect of Methionine Enkephalin on Tumor

作　　者：呼芳竹 陈灏 邓煜瑶 王晓楠 李胜军 单风平 HU Fang-zhu;CHEN Hao;DENG Yu-yao;WANG Xiao-nan;LI Sheng-jun;SHAN Feng-ping(Teach.&Res.Div.of Immunol.,Schl.of Basic Med.Sci.,China Med.Uni,Shengyang 110122)

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110122

出　　处：《微生物学杂志》2021年第2期53-59,共7页Journal of Microbiology

基　　金：国家自然科学基金项目(31670921)。

摘　　要：蛋氨酸脑啡肽(Methionine enkephalin, MENK)是一种内源性阿片肽,有良好的抗肿瘤作用,而肠道菌群对MENK抗肿瘤作用的影响尚不清楚。建立小鼠肠道菌群紊乱模型及荷瘤小鼠模型,以16S rRNA测序法检测肠道菌群丰度、监测小鼠体重、绘制肿瘤生长曲线,以流式细胞术检测小鼠肿瘤浸润CD4^(+)T细胞、CD8^(+)T细胞、Treg细胞百分比。结果表明,小鼠肠道菌群紊乱模型建立成功;肠道菌群正常与紊乱荷瘤小鼠同时接受MENK治疗,后者肿瘤体积更大,菌群正常荷瘤小鼠肿瘤浸润的CD4^(+)T细胞、CD8^(+)T细胞百分比上调,Treg细胞百分比下调,而菌群紊乱荷瘤小鼠与菌群正常荷瘤小鼠相比,肿瘤浸润CD4^(+)T细胞、CD8^(+)T细胞百分比下调,Treg细胞百分比上调。该研究表明肠道菌群紊乱可以降低MENK治疗肿瘤的疗效。Methionine enkephalin(MENK) is an endogenous opioid peptide that has good antitumor effects, while the influence of intestinal microbial population(IMP) on the anti-tumor effect of MENK is still unclear. In this study, a mouse IMP disorder model and a tumour-bearing mouse model were established to detect the IMP abundance by 16 S rRNA sequencing method, to monitor the weight of mice, and draw tumor growth curve, and detect the percentage of CD4^(+)T cells, CD8^(+)T cells and Treg cells infiltrated by mice tumor by flow cytometry. The results showed that the model of IMP disturbance in mice was established successfully. Both tumor-bearing mice with normal IMP and tumor-bearing mice with disturbed IMP were treated with MENK, the latter had a larger tumor volume, the percentage of CD4^(+)T cells and CD8^(+)T cells infiltrated by tumor in tumor-bearing mice with normal IMP was up-regulated, Treg cells percentage was down-regulated, when tumor-bearing mice with disordered IMP were compared with tumor-bearing mice with normal IMP, the percentage of CD4^(+)T cells and CD8^(+)T cells infiltrated by tumor in tumor-bearing mice was down-regulated, Treg cells percentage was up-regulated. This study showed that IMP disturbance could reduce the efficacy of MENK in treatment of tumors.

关 键 词：肠道菌群 蛋氨酸脑啡肽 肿瘤治疗 小鼠肉瘤S180 肿瘤浸润淋巴细胞 

分 类 号：Q939.93[生物学—微生物学]