参红通络方对大鼠动脉粥样硬化斑块的影响  

Effect of Shenhong Tongluo Prescription on Atherosclerotic Plaque

作　　者：靳宏光 朱星 李铁[2] 王义强[1] 成光宇[3] JIN Hongguang;ZHU Xing;LI Tie;WANG Yiqiang;CHENG Guangyu(Heart Disease Center of Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China;Institute of Acupuncture and Massage,Changchun University of Chinese Medicine,Changchun 130117,China;Chinese Medicine Research Center of Affiliated Hospital,Changchun University of Traditional Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学附属医院心病中心,长春130021 [2]长春中医药大学针灸推拿研究所,长春130117 [3]长春中医药大学附属医院中医药研究中心,长春130021

出　　处：《医药导报》2021年第7期863-869,共7页Herald of Medicine

基　　金：吉林省自然科学基金资助项目(20160101153JC);长春中医药大学高层次人才基金资助项目(2018KJ23);吉林省教育厅“十三五”科学技术项目(JJKH20200884KJ)。

摘　　要：目的观察参红通络方对动脉粥样硬化大鼠动脉斑块的影响。方法将80只雄性SD大鼠随机分为假手术组、模型对照组、中药大剂量组、中药小剂量组和辛伐他汀组,每组16只。除假手术组外,其他组制备动脉粥样硬化模型。造模成功后,中药小剂量组、中药大剂量组分别给予参红通络方免煎农本颗粒7.15和28.60g·kg^(-1)·d^(-1)灌胃;辛伐他汀组给予辛伐他汀1 mg·kg-1·d-1灌胃;假手术组和模型对照组给予等容积0.9%氯化钠溶液灌胃。均每天1次,连续8周。酶联免疫吸附测定(ELISA)法检测氧化低密度脂蛋白(ox-LDL)、可溶性CD40配体(sCD40L)水平,苏木精-伊红(HE)染色观察颈总动脉组织病理学变化,免疫组化法观察斑块细胞间黏附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-α)表达,荧光定量聚合酶链反应(PCR)法检测动脉粥样硬化斑块内CD40mRNA及白细胞介素-1β(IL-1β)mRNA表达水平。结果与模型对照组比较,中药大剂量组与中药小剂量组ox-LDL、sCD40L含量下降(P<0.05,P<0.01),斑块中ICAM-1、TNF-α表达显著减少;颈总动脉HE染色结果显示,与模型对照组比较,中药各剂量组颈总动脉内膜光滑,平滑肌细胞排列规整,泡沫细胞蓄积和平滑肌增生减轻,中药大剂量组大鼠斑块内CD40mRNA与IL-1βmRNA表达显著降低(P<0.01)。结论参红通络方具有较好的抑制炎症因子、抗动脉粥样硬化、稳定动脉斑块作用,该作用可能通过干预CD40/CD40L信号转导实现。Objective To observe the effect of Shenhong Tongluo Prescription on aortic plaque in atherosclerotic rats.Methods A total of 80 male SD rats were randomly divided into sham operation group,model control group,high-dose group,low-dose group and simvastatin group with 16 rats in each group.Atherosclerosis model was prepared in other groups except for sham operation group.After successful modeling,the low-dose group and the high-dose group were given fried-free granule of Shenhong Tongluo Prescription 7.15 and 28.60 g·kg^(-1)·d^(-1)by gavage(dissolved in 2 mL warm water),respectively.The simvastatin group was given simvastatin 1 mg·kg^(-1)·d^(-1)by gavage(dissolved in 2 mL warm water).The sham operation group and model control group were given the same volume 0.9%sodium chloride solution once a day for 8 weeks.Enzyme-linked immunosorbent(ELISA)method was used for detection of oxidized low density lipoprotein(ox-LDL),soluble CD40 ligand(sCD40L)levels.The hematoxylin-eosin(HE)staining was used to observe the histopathological changes in common carotid arteries.The immunohistochemical method to observe intercellular adhesion molecule-1(ICAM-1),tumor necrosis factor alpha(TNF-α),quantitative polymerase chain reaction(qPCR)method to detect atherosclerotic plaque inside CD40 mRNA and interleukin 1 beta(IL-1β)mRNA expression level in the plaque.Results Compared with the model control group,the contents of ox-LDL and sCD40L in the high-dose group and the low-dose group decreased(P<0.05 and P<0.01).And the expressions of ICAM-1 and TNF-αin the plaque were significantly reduced.HE staining results of common carotid artery showed that the intima was relatively smooth and smooth muscle cells arranged relatively regularly after the intervention of traditional Chinese medicine.Accumulation of smooth foam cells and smooth muscle hyperplasia;high dose group can significantly reduce the expression of CD40 mRNA and IL-1βmRNA in plaques of rats(P<0.01).Conclusion Shenhong Tongluo Prescription has good effects on inhibiting inf

关 键 词：参红通络方 动脉粥样硬化 斑块 CD40/CD40L 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]