球等鞭金藻培养条件的响应面法优化  

Optimization of culture conditions of Isochrysis galbana by response surface method

作　　者：田镇 曹奕[1] 陈爱华[1] 吴杨平[1] 张雨[1] 陈素华 张志东 李秋洁 TIAN Zhen;CAO Yi;CHEN Aihua;WU Yangping;ZHANG Yu;CHEN Suhua;ZHANG Zhidong;LI Qiujie(Jiangsu Institute of Marine Fisheries,Nantong Jiangsu 226007,China;National Demonstration Center for Experinental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]江苏省海洋水产研究所,江苏南通226007 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《海洋渔业》2021年第3期377-384,共8页Marine Fisheries

基　　金：江苏省渔业科技类重大项目(D2018-1);江苏省科技厅苏北专项(SZ-YC2018064);水产良种保种及更新-文蛤项目(2019-SJ-006);南通市基础科学研究项目(JC2018026);水产良种保种及更新-文蛤项目(2020-SJ-006)。

摘　　要：为探究球等鞭金藻(Isochrysis galbana)的最优培养条件,采用实验生态学方法和响应面法,以球等鞭金藻的比生长速率为指标,设置不同温度、盐度、接种量单因素实验及Box-Benhnken(BBD)模型多因素交叉实验。结果表明:1)在一定温度、盐度及接种量范围内,球等鞭金藻的比生长速率随着各因素的增大,呈先升高后下降的趋势;2)通过响应面BBD模型优化出球等鞭金藻的最佳培养条件为:温度26.38℃、盐度26.61、接种量1.29×10^(6)个·mL^(-1),此时的球等鞭金藻的比生长速率最大,为0.44 d^(-1)。Isochrysis galbana has the advantages of small size and fast reproduction.It has become one of the main micro-algae with great potential for green sewage treatment technology under the new concept.Because of its high nutritional value,it is often used as bait in the artificial breeding of bivalve shellfish such as Meretrix meretrix,Solen grandis,Sinonovacula constricta and so on.Response surface method is a combination of statistics and mathematics.It not only has the interaction in the orthogonal experiment,but also can use the mathematical model to get the functional relationship between the response and each variable,so it is an effective method to further predict the combination of all variable factors and get the optimal response surface value to solve scientific and production problems.Specific growth rate refers to the increased biomass of single-celled organisms per unit mass per unit time,which is an important parameter reflecting the growth rate of single-celled organisms such as microalgae and bacteria.The specific growth rate can not only accurately reflect the specific growth rate of microalgae in a certain time period,but also can comprehensively reflect the dynamic changes of the balance between microalgae growth and mortality.In order to optimize the culture conditions of Isochrysis galbana,using the method of experimental ecology and response surface(Box-Behnken mode),different experimental groups with different temperatures,salinities and initial inoculation levels were set up.Single factor experiment and multifactor experiment(Box-Benhnken model)were constructed to obtain the specific growth rate of Isochrysis galbana.Results showed that:1)In a certain range of temperature,salinity and initial inoculation level,the specific growth rate of Isochrysis galbana increased first and then decreased with the increase of various factors;2)The optimization of culture conditions of Isochrysis galbana respectively were:temperature 26.38℃,salinity 26.61,and initial inoculation level 1.29×10^(6) cell·mL^

关 键 词：球等鞭金藻 温度 盐度 接种量 比生长速率 响应面法优化 

分 类 号：S963.21[农业科学—水产养殖]