2种内生和附生橡藻属绿藻的胞外分泌物研究  

Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp

作　　者：邓茹茹 马帅[2] 谭德冠[2] 付莉莉[2] 张家明[2] 孙雪飘[2] 刘平怀[1] DENG Ruru;MA Shuai;TAN Deguan;FU Lili;ZHANG Jiaming;SUN Xuepiao;LIU Pinghuai(School of Chemical Engineering and Technology,Hainan University,Haikou 570228,China;Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Hainan Institute of Tropical Agricultural Resources,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Tropical Microbe Resources,Haikou 571101,China)

机构地区：[1]海南大学化学工程与技术学院,海口570228 [2]中国热带农业科学院热带生物技术研究所/中国热带农业科学院海南热带农业资源研究院/海南省热带微生物资源重点实验室,海口571101

出　　处：《浙江大学学报（农业与生命科学版）》2021年第3期363-370,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金(31700195,31800271)。

摘　　要：为探讨2种不同生活方式的内生和附生橡藻属(Heveochlorella)绿藻的胞外分泌物之间的差异,本实验通过测定内生海南橡藻(H.hainanensis)和附生王棕橡藻(H.roystonensis)的生物量和培养液pH,采用非靶向代谢组学分析方法对其胞外分泌物进行定性检测。结果表明:2种藻共被检测到141种胞外分泌物,其中海南橡藻拥有8种特有成分,王棕橡藻拥有6种特有成分;两者胞外分泌物中氨基酸及其衍生物、酮醇类等组分的相对含量占比相近,但有机酸的差异较明显。不加有机碳源培养15 d,内生的海南橡藻生长缓慢,培养液偏酸性,有机酸占比为3.28%;附生的王棕橡藻生长正常,培养液偏碱性,有机酸占比为2.04%。添加0.5%蔗糖作为有机碳源时,海南橡藻生物量由0.09 g/L增至3.87 g/L,有机酸占比增至4.64%,胺类及生物碱含量有所降低,培养液pH下降至4.70;王棕橡藻生物量由3.20 g/L增长到5.24 g/L,有机酸占比降至1.13%,胺类及生物碱含量有所增高,培养液pH上升至8.32。总之,2种橡藻属绿藻在培养过程中培养液的酸碱度变化相反;添加有机碳源能明显促进它们的生长繁殖,并促进海南橡藻有机酸的分泌,抑制王棕橡藻有机酸的分泌,使两者培养液酸化或碱化程度增强。本实验结果可为橡藻属的进化分析及其胞外分泌物的开发利用提供一定的参考。To investigate the differences of extracellular metabolites between endophytic algae of Heveochlorella hainangensis and epiphytic algae of Heveochlorella roystonensis,the biomass and pH of culture medium were analyzed,as well as the extracellular metabolites were qualitatively detected by non-targeted metabolomics analysis.The results showed that totally 141 compounds were detected in the culture media of the two algae species,of which eight and six ingredients specifically presented in the culture media of H.hainangensis and H.roystonensis,respectively.The relative contents of amino acids and their derivatives,ketone alcohols and other components in their extracellular metabolites were similar,but those of organic acids were remarkably different.After cultured for 15 d in the culture medium without exogenous organic carbon sources,the growth of H.hainangensis was slow,and the culture medium was slightly acidic,and the proportion of organic acids was 3.28%.However,the growth of H.roystonensis was normal,and the culture medium was alkaline,and the proportion of organic acids was 2.04%.When cultured in the media containing 0.5%sucrose for 15 d,the biomass of H.hainangensis increased from 0.09 g/L to 3.87 g/L;the proportion of organic acids increased to 4.64%;the contents of amines and alkaloids decreased;and the pH of culture medium decreased to 4.70.In the meantime,the biomass of H.roystonensis increased from 3.20 g/L to 5.24 g/L;the proportion of organic acids decreased to 1.13%;the contents of amines and alkaloids increased;and the pH of culture medium increased to 8.32.In conclusion,the change of pH in the culture media of the two species is opposite.The addition of organic carbon sources could significantly promote their growth and reproduction,promote the secretion of organic acids from H.hainangensis,inhibit the secretion of organic acids from H.roystonensis,thus enhance the degree of acidification or alkalization of the two cultures.The above results can provide a basis for the research on the evolution of

关 键 词：海南橡藻 王棕橡藻 胞外分泌物 非靶向代谢组学 

分 类 号：Q949.211[生物学—植物学]