空间贴壁细胞培养模块研制及特性测试  

Development and Characteristic Test of Space Adherent Cell Culture Module

作　　者：郭飞马 李明[1,2,3] 王小雪 李飞[1,2,3] GUO Feima;LI Ming;WANG Xiaoxue;LI Fei(Shenzhou Space Biotechnology Group,China Academy of Space Technology,Beijing 100080,China;Space Bioengineering Research Center of China Aerospace Science and Technology Corporation,Beijing 100080,China;Beijing Space Bioengineering Technology Research Center,Beijing 100080,China)

机构地区：[1]中国空间技术研究院航天神舟生物科技集团有限公司,北京100080 [2]中国航天科技集团有限公司空间生物工程研究中心,北京100080 [3]北京市空间生物工程技术研究中心,北京100080

出　　处：《载人航天》2021年第3期276-281,共6页Manned Spaceflight

基　　金：中国航天科技集团有限公司自主研发项目(HTKJZY201801)。

摘　　要：针对空间环境的特殊性以及空间站资源有限性,研制一种空间细胞培养模块,使其适合在空间飞行或空间站进行细胞培养。根据空间搭载标准架构接口要求和细胞培养要求,提出模块的设计方案,包括培养室及密封设计;然后对模块的材料进行生物相容性筛选,并利用此模块培养骨肉瘤细胞(MG63),从骨肉瘤细胞的活力和凋亡两方面评价其培养效果。实验结果表明,研制的空间细胞培养模块操作简单,密封效果好,生物相容性佳。利用此模块培养MG63细胞72 h后,细胞生长状态和铺展性良好,细胞活性和凋亡与传统细胞培养瓶相比无显著性差异。可应用于空间飞行和空间站的细胞培养。According to the particularity of space environment and the limited resources of space station,a space cell culture module suitable for cell culture in spaceflight or onboard space station was developed.The design of culture chamber and sealing device were conducted on the basis to the requirements of space carrying interface and cell culture.Then,the biocompatibility of the materials was screened,and human osteosarcoma cells(MG63)were cultured using this module.The culture effect was evaluated from the viability and apoptosis of osteosarcoma cells.The experimental results showed that the space cell culture module was simple in operation,good in sealing and good in biocompatibility.After 72 h culture of MG63 cells using this module,the growth and spread of cells were fine,and there was no significant difference in cell activity and apoptosis as compared with cell culture flask.It may be applied to cell culture in space flight or onboard space station.

关 键 词：空间站 细胞培养 骨肉瘤细胞 培养模块 

分 类 号：R3186[医药卫生—生物医学工程] R854[医药卫生—基础医学]