红树林淡紫拟青霉EPS对HSV-1颅内感染小鼠脑组织NF-κB表达的影响  

Effects of extracellular polysaccharides from mangrove forest Paecilomyces Lilacinuson on expression of NF-κB in HSV-1 intracranial infection mice

作　　者：王永霞[1] 王娇娇 黄燕妮[1] 林英姿[1] WANG Yongxia;WANG Jiaojiao;HUANG Yanni;LIN Yingzi(Ministry of Education Key Laboratory of Tropical Disease of Hainan Medical University,Haikou,Hainan 571199,China)

机构地区：[1]海南医学院教育部热带病重点实验室,海口571109

出　　处：《重庆医学》2021年第12期1988-1992,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81560006);海南省重点研发项目(SZD180007);海南医学院省级及校级大学生创新创业项目(201811810046)。

摘　　要：目的探讨红树林淡紫拟青霉胞外多糖(EPS)对单纯疱疹病毒1型(HSV-1)颅内感染小鼠脑组织核因子κB(NF-κB)表达的影响。方法除阴性对照组外,其他各组小鼠均颅内注射病毒建立HSV-1颅内感染模型,次日起实验组分别经腹腔给予低剂量、中剂量和高剂量EPS干预,药物对照组用阿昔洛韦干预,病毒对照组和阴性对照组均用生理盐水干预,连续注射7 d。观察各组小鼠病变、死亡情况,免疫组织化学检测脑组织NF-κB表达情况,RT-qPCR检测脑组织NF-κB mRNA表达。结果除病毒对照组小鼠死亡率明显高于阴性对照组(P<0.05)外,其他各组间无明显差异(P>0.05)。免疫组织化学和RT-qPCR检测均发现,与病毒对照组相比,实验组小鼠脑组织NF-κB表达均有一定程度下调,其中高剂量EPS组下调最明显(P<0.05),低剂量、中剂量EPS组下调不明显(P>0.05)。低剂量、中剂量EPS组NF-κB表达与阴性对照组比较差异有统计学意义(P<0.05),高剂量EPS组与阴性对照组比较差异无统计学意义(P>0.05)。结论红树林淡紫拟青霉EPS具有一定抑制HSV-1颅内感染小鼠脑组织NF-κB表达的作用,且该抑制作用呈一定剂量依赖性。Objective To investigate the effects of the extracellular polysaccharides(EPS)isolated from mangrove forest Paecilomyces lilacinus on the expression of NF-κB in HSV-1 intracranial infection mice brain tissue.Methods Except for the negative control group,the other groups were intracranially injected with virus for constructing the HSV-1 intracranial infection model.The experiment group was given the low dose,middle dose and high dose EPS intervention,the medication control group used the acyclovir intervention,the viral control group and negative control group used the normal saline intervention.The injection consecutively lasted for 7 d.Then the mice lesions and death were observed in each group.The expression level of brain tissue NF-κB was detected by the immunohistochemical technique and the expression level of NF-κB mRNA was detected by RT-qPCR.Results Except the death rate in the viral control group was significantly higher than that in the negative control group,there was no significant difference among the other groups(P>0.05).The immunohistochemistry and RT-qPCR found that compared with the viral control group,the expression of brain tissue NF-κB in the experiment group had some degrees of down-regulation,in which the down regulation in the high dose EPS group was most significant(P<0.05),and the down regulation in the low dose and middle dose EPS groups was unobvious(P>0.05).The NF-κB expression level had significant differences in the low dose and middle dose EPS groups compared with the negative control group(P<0.05),while no significant the difference was found between the high dose EPS group and negative control group(P>0.05).Conclusion EPS from mangrove forest Paecilomyces lilacinus may be a potential candidate for inhibiting NF-κB expression in mice brain tissue with HSV-1 infection with a dose-dependent manner.

关 键 词：红树林淡紫拟青霉 胞外多糖 单纯疱疹病毒1型 核因子κB 

分 类 号：R931[医药卫生—生药学]