分光光度法中比色皿的光程长度对实验结果的影响——以海水中亚硝酸盐氮的测定为例  

Influence of Cuvette′s Optical Path Length on Experimental Results by Spectrophotometry:A Case Study of Taking the Determination of Nitrite Nitrogen in Seawater

作　　者：孙欢 谭晓璇 屠建波[1] 阚文静[1] SUN Huan;TAN Xiaoxuan;TU Jianbo;KAN Wenjing(Tianjin Marine Environmental Monitoring Central Station,SOA,Tianjin 300457,China;Oceanic Consulting Center,MNR,Beijing 100161,China)

机构地区：[1]国家海洋局天津海洋环境监测中心站,天津300457 [2]自然资源部海洋咨询中心,北京100161

出　　处：《海洋开发与管理》2021年第6期76-80,共5页Ocean Development and Management

基　　金：山东省海洋生态环境与防灾减灾重点实验室开放基金项目(201907).

摘　　要：分光光度法是常用的定量分析方法之一,被广泛应用于水环境监测实验中。文章以萘乙二胺分光光度法测定海水中亚硝酸盐氮为例,探索选用不同光程长度的比色皿测定吸光值对物质含量的影响,并通过数理统计方法对实验数据进行分析。研究结果表明:选用不同光程长度的比色皿测得水样中的物质含量存在差异,即比色皿的光程长度越小,测得的物质含量越高;在相同实验条件下,选用光程长度更小的比色皿,实验的准确性和校准曲线的线性均更好。Spectrophotometry is one of the commonly used quantitative analysis methods.It is widely used in water environment monitoring experiments.In this paper,the determination of nitrite nitrogen in seawater by the naphthalene ethylenediamine spectrophotometric method was a case study,which explored the differences of using cuvettes with different optical path lengths to determine the concentration of substances.The experimental data was analyzed by mathematical statistics.The results showed that the content of substance in samples were different because of different optical path length of cuvettes.The concentration was higher when the cuvette was shorter.In the same condition,the accuracy of the experiment and the linearity of the calibration curve were better when the cuvette was shorter.

关 键 词：分光光度法 比色皿 光程长度 吸光值 水环境监测 

分 类 号：P76[天文地球—海洋科学] X834[环境科学与工程—环境工程]