Development of an Artificial Diet for Effective Oral Delivery of dsRNA to Soybean Pod Borer,Leguminivora glycinivorella(Lepidoptera:Tortrici-dae)  

作　　者：Wang Guo-yue Wang Nan Li Ming-yue Xin Jun-jie Qin Yu-shi Meng Fan-li Yuan Qiang Wang Xiao-yun Zhang Xiao-ming 

机构地区：[1]Key Laboratory of Soybean Biology in the Chinese Ministry of Education,College of Agriculture,Northeast Agricultural University,Harbin 150030,China [2]College of Agriculture,Northeast Agricultural University,Harbin 150030,China [3]Division of Soybean Breeding and Seeds,Soybean Research&Development Center,CARS(Key Laboratory of Biology and Genetics&Breeding for Soybean in Northeast China,Ministry of Agriculture),Northeast Agricultural University,Harbin 150030,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2021年第2期15-22,共8页东北农业大学学报（英文版）

基　　金：Supported by the Harbin Science and Technology Innovation Program(2017RAQXJ122);China National Novel Transgenic Organisms Breeding Project(2016ZX08004-004-006);the Heilongjiang Natural Science Foundation(C2018012);the Chinese National Natural Science Foundation(31201229);Soybean Research&Development Center(CARS-04-PS04)。

摘　　要：RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The neonate larvae of SPB are endophagous,initially feeding on the inner layer of the pod and then on the immature soybean seeds,a behavior that makes them difficult to rear.Therefore,a suitable artificial diet is desirable to facilitate RNAi research.Seven artificial diets and soybean pods were used for rearing SPB neonate larvae.The survival and mean weight of the larvae reared on artificial diet 6(A6,included 2 g soybean flour and 10 g R3 immature soybean pod powder)were found to be significantly higher than those for larvae reared on all other diets.Subsequently,A6 diet was prepared in DEPC-treated water to obtain a RNase-free artificial diet(RF).The survival and mean weight of the larvae were not significantly different between A6 and RF diets.Feeding neonate larvae with RF-SpbP0 dsRNA(ribosomal protein P0)silenced SpbP0 gene,resulting in arrested development and increased mortality.The results of this study suggested that the newly developed A6 or RF diets could rear SPB neonate larvae and help towards developing an effective method for oral delivery of dsRNA to SPB larvae.

关 键 词：soybean pod borer artificial diet RNA interference 

分 类 号：S565.1[农业科学—作物学]