3-羟基根皮苷对小鼠血糖及糖异生关键酶G-6-Pase的影响  

Effects of 3-hydroxyphloridin on Blood Glucose and Gluconeogenesis Key Enzyme G-6-Pase in Mice

作　　者：汪鋆植[1] 边宇 邓改改[1,2] 吴翠 李澳 廖兴悦 黄永梅 WANG Junzhi;BIAN Yu;DENG Gaigai;WU Cui;LI Ao;LIAO Xingyue;HUANG Yongmei(Hubei Key Laboratory of Natural Products Research&Development,College of Biological&Pharma-ceutical Sciences,China Three Gorges Univ.,Yichang 443002,China;Medical College of China Three Gorges Univ.,Yichang 443002,China)

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室/湖北省土家族医药研究所,湖北宜昌443002 [2]三峡大学医学院,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2021年第4期106-112,共7页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(81903769);湖北省技术创新专项(民族专项)(2018AKB029)。

摘　　要：探究湖北海棠(Malus hupehensis(Pamp.) Rehd.)叶中主要成分3-羟基根皮苷对小鼠血糖及糖异生关键酶葡萄糖-6-磷酸酶(glucose-6-phosphatase, G-6-Pase)的影响,为稳定血糖提供可行方案.采用葡萄糖氧化酶法测定胰岛素抵抗模型HepG2细胞的葡萄糖摄取;注射链脲佐菌素建立二型糖尿病小鼠模型,血糖试纸测定小鼠糖耐量、空腹血糖及餐后血糖;蛋白印迹法检测G-6-Pase的表达.结果表明:3-羟基根皮苷增强了胰岛素抵抗细胞模型对葡萄糖的摄取;3-羟基根皮苷增强了不同健康状况下,小鼠的糖耐受能力,升高正常小鼠空腹血糖水平,降低糖尿病小鼠空腹血糖水平,降低正常小鼠及糖尿病小鼠餐后血糖水平;高血糖水平时,抑制G-6-Pase表达,低血糖水平时增加G-6-Pase表达.因此,3-羟基根皮苷不仅具有降低糖尿病小鼠血糖的作用,还具有稳定血糖,减少血糖波动的作用,其机制与双向调节G-6-Pase表达相关.The effect of 3-hydroxyphloridin, main component of Malus hupehensis leaves, on blood glucose(BG) and glucose-6-phosphatase(G-6-Pase) in mice is investigated to provide the feasible scheme for stabilizing the BG. The glucose uptake of insulin resistance HepG2 cells is measured by the method of glucose oxidase. The mice model of type 2 diabetes is established by the injection of streptozotocin. Meanwhile, the glucose tolerance, fasting blood glucose(FBG) and postprandial blood glucose(PBG) are determined by BG test strips. The expression of G-6-Pase is detected by Western blot. The results show that the glucose uptake is enhanced and the expression of G-6-Pase is inhibited by 3-hydroxyphloridzin in insulin resistance HepG2 cells. The glucose tolerance in mice under different health conditions is enhanced by 3-hydroxyphloridin. The FBG level in normal mice is increased, which in diabetic mice is reduced, and the PBG level in the normal and diabetic mice is reduced. Furthermore, the expression of G-6-Pase is inhibited when BG is high, otherwise, the expression of G-6-Pase is increased. Therefore, it is the conclusion that BG in diabetic mice is reduced by 3-hydroxyphloridin. Meanwhile, the BG is stabilized to reduce the BG fluctuation. The mechanism is related to the bidirectional regulation of G-6-Pase expression.

关 键 词：3-羟基根皮苷 糖尿病 G-6-Pase 血糖稳定 湖北海棠 

分 类 号：R965[医药卫生—药理学]