HPLC-QAMS法同时测定芪冬颐心颗粒中7种成分的含量  

Simultaneous Determination of Seven Active Components in Qidong Yixin Granules by HPLC-QAMS

作　　者：刘华 乔春凤 陈海燕[2] LIU Hua;QIAO Chunfeng;CHEN Haiyan(Department of Pharmacy,Zhengzhou Yihe Hospital,Zhengzhou 450047,China;Department of Pharmacy,Guangxi Medical College,Nanning 530023,China)

机构地区：[1]郑州颐和医院药学部,郑州450047 [2]广西卫生职业技术学院药学系,南宁530023

出　　处：《药学与临床研究》2021年第3期179-182,共4页Pharmaceutical and Clinical Research

基　　金：广西壮族自治区教育厅职业教育教学改革立项项目(GXGZJG2015B237)。

摘　　要：目的:建立同时测定芪冬颐心颗粒中毛蕊异黄酮葡萄糖苷、芒柄花素、朝藿定A、淫羊藿苷、宝藿苷I、去氢土莫酸、茯苓酸含量的高效液相色谱一测多评(HPLC-QAMS)法。方法:采用HPLC-QAMS法,色谱柱采用Agilent TC-C18,柱温为30℃;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;流速为1.0 mL·min^(-1);检测波长分别为254、270和210 nm。以淫羊藿苷为内参物,测定其与毛蕊异黄酮葡萄糖苷、芒柄花素、朝藿定A、宝藿苷I、去氢土莫酸、茯苓酸的相对校正因子,利用该相对校正因子计算各成分含量,同时采用外标法(ESM)测定7种成分含量,比较一测多评法和外标法含量测定结果的差异。结果:7种成分在各自范围内线性关系良好;平均加样回收率及相对标准偏差(RSD)分别为97.44%(0.93%)、99.60%(0.86%)、99.21%(1.36%)、100.03%(0.75%)、98.77%(1.08%)、97.64%(1.22%)、96.86%(1.07%);一测多评法计算值和外标法实测值无显著差异。结论:所建立的HPLC-QAMS法可用于芪冬颐心颗粒中7种成分的含量测定,为芪冬颐心颗粒的质量控制提供科学的依据。Objective:To establish an HPLC-QAMS method for simultaneous determination of calycosin7-O-β-D-glucopyranoside,formononetin,epimedin A,icariin,baohuosideⅠ,dehydrotumulosic acid and pachymic acid in Qidong Yixin Granules.Methods:An HPLC-QAMS method was applied using an Agilent TC-C18 column,with the mobile phase consisted of acetonitrile-0.1%phosphoric acid aqueous solution eluting at 1.0 mL·min^(-1) gradiently,and the column temperature was maintained at 30℃.The detection wavelengths were set at 254,270 and 210 nm.Icariin was selected as the internal reference substance,and the relative correlation factors(RCF)of calycosin7-O-β-D-glucopyranoside,formononetin,epimedin A,baohuosideⅠ,dehydrotumulosic acid and pachymic acid were determined,and their contents were calculated by RCF.The results of content determination by QAMS were compared with those by external standard method(ESM).Results:The seven components showed good linear relationships within their own ranges,with average recoveries(RSDs)at 97.44%(0.93%),99.60%(0.86%),99.21%(1.36%),100.03%(0.75%),98.77%(1.08%),97.64%(1.22%)and 96.86%(1.07%),respectively.The quantitative results of calycosin7-O-β-D-glucopyranoside,formononetin,epimedin A,icariin,baohuosideⅠ,dehydrotumulosic acid and pachymic acid obtained by QAMS were similar to those obtained by ESM.Conclusion:The HPLC-QAMS method is suitable for the determination of seven active ingredients in Qidong Yixin Granules,which can be used for the quality control of Qidong Yixin Granules.

关 键 词：高效液相色谱一测多评法 芪冬颐心颗粒 毛蕊异黄酮葡萄糖苷 芒柄花素 朝藿定A 淫羊藿苷 宝藿苷I 去氢土莫酸 茯苓酸 

分 类 号：R927.2[医药卫生—药学]