天津空港经济区枣疯病植原体的分子鉴定  

Molecular Identification of Phytoplasma Associated with Jujube Witchs’ Broom in Tianjin Air Port Economic Area

作　　者：唐芳 牛春敬 王仲敏 冯洁 廖芳 高建 胡佳续 TANG Fang;NIU Chun-Jing;WANG Zhong-Min;FENG Jie;LIAO Fang;GAO Jian;HU Jia-Xu(Tianjin Custom Animal Plant and Foodstuffs Inspection Center,Tianjin 300461)

机构地区：[1]天津海关动植物与食品检测中心,天津300461

出　　处：《中国口岸科学技术》2021年第5期4-8,共5页China Port Science and Technology

基　　金：国家重点研发计划国家质量基础(NQI)课题(2017YFF0210305)。

摘　　要：利用分子生物学技术对天津空港经济区枣疯病样品进行分类鉴定。采用植原体16S rDNA通用引物R16mF2/R16mR1对患病植株总DNA进行PCR扩增,得到约1.4 kb特异性片段。经克隆测序、Blast比对和系统进化树构建分析,结果表明,天津空港枣疯病植原体16S rDNA基因片段长1432 bp,与国内枣疯病植原体Jinsimizao株系相似性最高,达99.93%,归属于16SrⅤ组(Elm yellows group,榆树黄化组)。本研究首次在天津空港地区检测到了枣疯病植原体,可为植原体病害流行学分析以及病害防治提供参考。The pathogenic agent of jujube witches’ broom(JWB-TJKG) collected from Tianjin air port economic area was identified by molecular biology. Universal phytoplasma primer pairs R16 mF2/R16 mR1, were used to amplify 16 S rDNA by PCR, specific fragment about 1.4 kb was yielded in the end. After cloning andsequencing, blast searching and phylogenetic tree analysis, the result showed that the JWB-TJKG consists of 1432 bp in 16 S rDNA gene, which shared the highest similarity(99.93%) with the strain of JWB-Jinsimizao, and belongs to Elm yellows group(16 Sr Ⅴ group). This paper is the first report to detect jujube witches’ broom in Tianjin air port economic area, which provides a reference for epidemic analysis and prevention of phytoplasma disease.

关 键 词：枣疯病 植原体 分子鉴定 16S rDNA 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]