1-磷酸鞘氨醇受体2(S1PR2)通过保护肠黏膜屏障功能缓解炎症性肠病的作用  

Effect of 1-phosphossphingosine receptor 2(S1PR2)on relieving inflammatory bowel disease by protecting intestinal mucosal barrier function

作　　者：薛海波[1] 宋冰欣 丁莉 马丽丽[2] 潘贻飞[3] 陈坛辀[1] XUE Haibo;SONG Bingxin;DING Li;MA Lili;PAN Yifei;CHEN Tanzhou(Department of Gastroenterology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China;Department of Rheumatology and Immunology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China;Department of Colorectal and Anal Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第一医院消化内科,浙江温州325000 [2]温州医科大学附属第一医院风湿免疫科,浙江温州325000 [3]温州医科大学附属第一医院结直肠肛门外科,浙江温州325000

出　　处：《中国现代医生》2021年第15期32-36,共5页China Modern Doctor

基　　金：浙江省自然科学基金项目(LY19H160022);浙江省温州市科技局项目(Y20170057)。

摘　　要：目的研究1-磷酸鞘氨醇受体2(S1PR2)通过保护肠黏膜屏障缓解炎症性肠病的作用。方法构建S1PR2基因敲除鼠。用硫酸葡聚糖钠盐(DSS)诱导结肠炎。血清荧光素异硫氰酸酯(FITC)浓度测定体内外小鼠肠道渗透性。酶联免疫吸附实验(ELISA)测定细胞悬液中的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)水平。结果野生型(WT)小鼠存活率及体重高于S1PR2-/-小鼠,差异有统计学意义(P<0.05)。S1PR2-/-小鼠的结肠缩短比WT小鼠更明显,差异有统计学意义(P<0.05)。DSS处理组小鼠结肠细胞悬液中IFN-γ、IL-6和TNF-α浓度较对照组水平升高,差异有统计学意义(P<0.05)。结论S1PR2通过保护肠黏膜屏障功能缓解炎症性肠病。Objective To investigate the effects of 1-phossphingosine receptor 2(S1PR2)on relieving inflammatory bowel disease(IBD)by protecting the intestinal mucosal barrier.Methods S1PR2 gene knockout mice were constructed.Dextran sulfate sodium salt(DSS)was used to induce colitis.Serum fluorescein isothiocyanate(FITC)concentration was measured for intestinal permeability of mice in vitro and in vivo.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interferon-γ(IFN-γ)in cell suspensions were determined by enzyme-linked immunosorbent assay(ELISA).Results The survival rate and body weights of wild-type(WT)mice were higher than those of S1PR2-/-mice,and the differences were statistically significant(P<0.05).The concentrations of IFN-γ,IL-6 and TNF-αin colon cell suspension of mice in the DSS group were higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion S1PR2 alleviates inflammatory bowel disease by protecting intestinal mucosal barrier function.

关 键 词：1-磷酸鞘氨醇 1-磷酸鞘氨醇受体2 肠黏膜屏障功能 炎症性肠病 

分 类 号：R331.3[医药卫生—人体生理学]