在角质细胞和EpiSkin表皮模型上测试化妆品原料对CD44蛋白表达的影响  

The Effects of Cosmetic Ingredients on CD44 Protein Expression Were Tested on Keratinocytes and EpiSkin Models

作　　者：周明 王萍 朱炎[1] 裘捷 丁春梅 王伟 ZHOU Ming;WANG Ping;ZHU Yan;QIU Jie;DING Chunmei;WANG Wei(School of life sciences,Fudan University,Shanghai 200433;L'Oreal R&I Center,Shanghai 201206)

机构地区：[1]复旦大学生命科学学院,上海200433 [2]欧莱雅研发与创新中心,上海201206

出　　处：《生物化工》2021年第3期26-29,34,共5页Biological Chemical Engineering

摘　　要：目的:测试化妆品原料对角质细胞和皮肤模型中CD44蛋白表达的影响。方法:视黄酸(RA)、泛醇(ProVB5)、透明质酸(HA)3种原料单独或者ProVB5和HA联合配伍加入人角质细胞或EpiSkin表皮模型培养基中培养处理48 h;其中配伍组在模型上增加了局部表皮涂抹处理,采用免疫荧光法检测样品中CD44蛋白的表达量。结果:在人角质细胞测试中,ProVB5、HA单独处理组和联合配伍组的CD44蛋白表达均有显著增加,但这种现象未在表皮模型中观察到;RA对表皮模型有显著增厚的作用。结论:CD44蛋白在二维和三维体系中的表达存在差异,RA对表皮模型具有显著的增厚作用。Objective:To test the effect of cosmetic materials on the expression of CD44 protein in keratinocytes and skin models.Methods:Retinoic Acid(RA),Panthenol(ProVB5)and Hyaluronic Acid(HA)alone or in combination with ProVB5 and HA were added into human keratinocytes or EpiSkin epidermal model medium for 48h;In the compatibility group,topical treatment was added to the epidermal model,and the expression of CD44 protein in the samples was detected by immunofluorescence.Results:In human keratinocyte test,the expression of CD44 protein in ProVB5,HA alone and combined groups were significantly increased,but this phenomenon was not observed in the epidermal model;RA has a significant thickening effect on the epidermal model.Conclusion:The expression of CD44 protein is different between two-dimensional and three-dimensional systems.RA has a significant thickening effect on the epidermal model.

关 键 词：EpiSkin表皮模型 CD44蛋白 透明质酸 视黄酸 人角质细胞 

分 类 号：TQ658[化学工程—精细化工]