基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用  

Application of Fluorescent Probe Based on Dicyanoisophorone in Detection of Thiophenol

作　　者：张改清 阴彩霞[2] ZHANG Gai-Qing;YIN Cai-Xia(Department of Chemistry and Chemical Engineering,Lüliang University,Lüliang,Shanxi 033000,China;Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Molecular Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]吕梁学院化学化工系,吕梁033000 [2]山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《无机化学学报》2021年第7期1245-1250,共6页Chinese Journal of Inorganic Chemistry

基　　金：山西省高等学校教学改革创新项目(No.J2018192);国家自然科学基金(No.21775096,22074084)资助。

摘　　要：为了开发具有高选择性和高灵敏度检测苯硫酚的方法,我们基于二氰基异佛尔酮染料优异的光学性能及良好的膜渗透性,以二氰基异佛尔酮衍生物作为荧光团,引入强吸电子基2,4⁃二硝基苯合成荧光探针(YC1)用于特异性识别苯硫酚。该探针对苯硫酚显示出明显的红色荧光(发射波长为594 nm,激发波长为420 nm),具有荧光增强(turn⁃on)的性质。探针对苯硫酚的识别具有良好的选择性且在PBS/DMSO(PBS=磷酸缓冲盐溶液,DMSO=二甲亚砜)的体系中检测限为0.65μmol·L^(-1)。此外,该探针已成功应用于HeLa细胞内4⁃甲基苯硫酚的成像。To develop a method with high selectivity and sensitivity to detect thiophenol,based on the excellent optical properties and good membrane permeability of dicyanoisophorone dyes,we chose dicyanoisophorone derivatives as fluorophores and introduced a strong electron⁃withdrawing 2,4⁃dinitrobenzene synthetic fluorescent probe(YC1)for the specific recognition of thiophenol.The probe showed obvious red fluorescence on thiophenol(emission wavelength:594 nm,excitation wavelength:420 nm)and had a fluorescence⁃enhanced(turn⁃on)property for the recognition of thiophenol.The probe had a good selectivity for the recognition of thiophenol and the detection limit of the probe was 0.65μmol·L^(-1) in PBS/DMSO system(PBS=phosphate buffer saline,DMSO=dimethyl sulfoxide).In addition,the probe has been successfully applied to the fluorescence imaging of 4⁃methylthiophenol in HeLa cells.

关 键 词：异佛尔酮 苯硫酚 荧光增强 生物成像 

分 类 号：TQ617.3[化学工程—精细化工]