高盐度复杂染料废水脱色菌群的降解特性  

Biodegradation characteristics of high-salinity complex dyes wastewater with high salinity by the bacterial consortium

作　　者：李进家 范晓丹 张道虹 袁正通 周佳滢 王雪琦 LI Jin-jia;FAN Xiao-dan;ZHANG Dao-hong;YUAN Zheng-tong;ZHOU Jia-ying;WANG Xue-qi(Tianjin Key Laboratory of Water Aquatic Science and Technology,School of Environmental and Municipal Engineering,Tianjin Chengjian University,Tianjin 300384,China)

机构地区：[1]天津城建大学环境与市政工程学院,天津水质科学与技术重点实验室,天津300384

出　　处：《中国环境科学》2021年第6期2631-2638,共8页China Environmental Science

基　　金：国家自然科学基金(51808378)。

摘　　要：从天津市市政污泥中筛选出复杂染料脱色菌群,其经16SrDNA基因序列鉴定,主要由Dokdonella(12.26%)、Dyella(10.19%)、Saccharibacteria genera(7.07%)、Rhodobacter(5.80%)、Pseudodoxanthomonas(3.61%)、Flavihumibacter(3.02%)组成.将其用于高盐度复杂染料废水的降解脱色,并研究共代谢基质和各种理化参数对染料废水脱色的影响.结果表明,最佳共代谢基质为葡萄糖和硫酸铵;最佳脱色条件:温度为35℃、pH值为9.0、菌群接种浓度为1.2g/L;在高盐度条件下(50g/L),菌群能高效脱色复杂染料废水,72h脱色率达到(87.0±2.4)%.经高效液相色谱仪(HP-LC)、傅里叶红外光谱(FT-IR)及气相色谱质谱联用仪(GC-MS)分析,甲基橙首先被迅速降解,分散蓝次之,酸性品红的降解较慢;复杂染料的发色基团(-N=N-,-NH_(2),-SO_(3))和苯环结构被破坏;其降解产物为苯胺、乙酰胺及2-氨基-5-甲基苯甲酸等.In this study,a bacterial consortium was isolated from municipal activated sludge from Tianjin,China.Identification via 16SrDNA sequencing showed that it was mainly consisted of Dokdonella(12.26%),Dyella(10.19%),Sacharibacteria genera(7.07%),Rhodobacter(5.80%),Pseudodoxanthomonas(3.61%),and Flavihumibacter(3.02%).The decolorization conditions of high-salinity complex dyes wastewater,such as co-metabolic substrates as well as various physical and chemical parameters,were studied.The results showed that the best co-metabolic substations for the decolorization of complex dyes wastewater were glucose and ammonium sulfate.The optimum decolorization conditions were 35℃,pH 9.0,and inoculum concentration of 1.2g/L.Under the condition of high salinity(50g/L),the decolorization efficiency of complex dyes wastewater reached(87±2.4)%within 72h.Methyl orange was degraded first,followed by disperse blue,while acid fuchsin had a slow degradation rate.The chromophore groups(-N=N-,-NH_(2),and-SO_(3))and the benzene rings of complex dyes were destroyed,as identified with by high performance liquid chromatography and Fourier transform infrared spectroscopy.Degradation metabolites included aniline,acetamide,and 2-amino-5-methylbenzoic acid as identified by gas chromatography mass spectrometry.

关 键 词：复杂染料 高盐度 菌群 生物降解 

分 类 号：X511[环境科学与工程—环境工程]