磷掺杂ZIF-67衍生复合材料的免疫传感器检测人免疫球蛋白  

Immunosensor based on phosphorus-doped ZIF-67 derivative composite for detection of human immunoglobulin

作　　者：唐皇聪 张捷[1] 关丽 张岩[1] 董社英[1] TANG Huangcong;ZHANG Jie;GUAN Li;ZHANG Yan;DONG Sheying(School of Chemistry and Chemical Engineering,Xi’an University of Architecture and Technology,Xi’an 710055)

机构地区：[1]西安建筑科技大学化学与化工学院,西安710055

出　　处：《分析试验室》2021年第6期649-653,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金青年项目(21807086);国家自然科学基金面上项目(21575111)资助。

摘　　要：以六水合硝酸钴(Co(NO_(3))_(2)·6H_(2)O)和2-甲基咪唑(C4H6N2)为原料,通过水热法合成了ZIF-67,进一步煅烧后制备磷掺杂的CoP@C,并对其形貌及物相进行表征。CoP@C与离子液体(IL)混合修饰碳糊电极(CPE)制备CoP@C/IL-CPE,并以戊二醛(GA)为交联剂,牛血清白蛋白(BSA)为封闭剂,构建BSA/免疫球蛋白抗体(anti-IgG)/GA/CoP@C/IL-CPE传感器。通过循环伏安法和电化学阻抗谱研究其电化学行为,并用差分脉冲伏安法对人免疫球蛋白进行定量检测。在最佳条件下,该传感器对免疫球蛋白检测的线性范围为0.01~1000 ng/mL,检出限为4 pg/mL。ZIF-67 was prepared by hydrothermal method using cobalt nitrate hexahydrate(Co(NO_(3))_(2)·6H_(2)O)and 2-methylimidazole(C4 H6 N2)as raw materials.After further calcination,phosphorus-doped CoP@C was synthesized.The morphology and phase of the materials were characterized.Moreover,CoP@C/IL-CPE was constructed by mixing CoP@C with ionic liquid(IL)to modify the carbon paste electrode(CPE).Then,by using glutaraldehyde(GA)as a cross-linking agent and bovine serum albumin(BSA)as a blocking agent,a BSA/anti-IgG/GA/CoP@C/IL-CPE sensor was obtained.Cyclic voltammetry and electrochemical impedance spectroscopy were used to study their electrochemical behavior,and differential pulse voltammetry was used to quantitatively detect human IgG.Under optimal conditions,the fabricated biosensor exhibited an outstanding electrochemical performance in the selective and sensitive measurement of IgG in the linear range of 0.01-1000 ng/m L with an extremely low detection limit of 4 pg/m L.

关 键 词：CoP@C 水热法 复合材料 免疫球蛋白 电化学免疫传感器 

分 类 号：O646[理学—物理化学]