614例急性腹泻患者诺如病毒基因型分析  

Genotyping of norovirus in 614 patients with acute diarrhea

作　　者：姚秀萍 李凌云[1] 吕雷立[1] YAO Xiu-ping;LI Ling-yun;LV Lei-li(Clinwal Laboratory,Lishui Peoples Hospital,Lishui,Zhejiang 323000,China)

机构地区：[1]丽水市人民医院检验科,浙江丽水323000

出　　处：《中国卫生检验杂志》2021年第10期1204-1207,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科技计划(2018KY923)。

摘　　要：目的 分析急性腹泻患者中诺如病毒的感染情况及流行基因型。方法 采用实时荧光定量PCR(RT-q PCR)对2014年-2019年丽水市人民医院614例急性腹泻患者的粪便标本进行诺如病毒的检测。结果 诺如病毒的检出率为19. 22%(118/614),其中性别差异无统计学意义(χ^(2)=1. 408,P> 0. 05);基因型以GⅡ型为主,其中GⅠ型和GⅡ型单独感染以及两者共同感染的检出率分别为0. 98%(6/614)、16. 78%(103/614)和1. 47%(9/614);感染对象主要为<15岁的儿童与少年,检出率为35. 81%(77/215),各年龄组检出率差异有统计学意义(χ^(2)=61. 68,P <0. 000 1);感染存在明显的季节性,每年11月为感染高峰期,且各月份检出率差异有统计学意义(χ^(2)=76. 12,P <0. 000 1)。结论 应在秋季中旬至冬季中旬,加强GⅡ型诺如病毒在丽水地区儿童群体中的分子流行病学监测与防控工作。Objective To analyze the infection and epidemic genotype of norovirus in patients with acute diarrhea.Methods Detection of norovirus was conducted in stool samples of 614 suspected viral diarrhea patients in Lishui City from 2014 to 2019 by real-time fluorescence quantitative PCR(RT-PCR).Results The detection rate of norovirus was 19.22%(118/614),with the difference not statistically significant between genders(χ^(2)=1.408,P>0.05).The genotypes were mainly GII.The detection rates of GI and GII infection alone and co-infection were 0.98%(6/614),16.78%(103/614)and 1.47%(9/614),respectively.The main target of infection was children and adolescents under 15 years,and the detection rate was 35.81%(77/215).There was statistical significance on the difference in the detection rate among different age groups(χ^(2)=61.68,P<0.0001).There was obvious seasonality in the infection,with the high incidence in November,and the difference on detection rate was statistically significant between different months(χ^(2)=76.12,P<0.0001).Conclusion From mid-autumn to mid-winter,we should strengthen the molecular epidemiological surveillance and control of GII Norovirus among children in Lishui area.

关 键 词：诺如病毒 急性腹泻 感染 儿童 

分 类 号：R446.5[医药卫生—诊断学]