丹参饮介导过氧化物酶体增殖物激活型受体γ减轻大鼠冠脉粥样硬化  

The Mechanism Study of Danshen Decoction Mediated PPAR-γto Reduce Coronary Atherosclerosis in Rats

作　　者：祝雪丽[1] 熊尚全[3] 陈怀宇[3] 褚剑锋 吴翔[1] 何绍吾 李玮[1] ZHU Xueli;XIONG Shangquan;CHEN Huaiyu;CHU Jianfeng;WU Xiang;HE Shaowu;LI Wei(Department of Critical Care Medicine,People′s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China;Institute of Integrated Chinese and Western medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China;Department of Internal Medicine,First Clinical Medical College,Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China)

机构地区：[1]福建中医药大学附属人民医院重症医学科,福州350004 [2]福建中医药大学中西医结合研究院,福州350108 [3]福建中医药大学第一临床医学院内科教研室,福州350004

出　　处：《世界中医药》2021年第11期1698-1701,1707,共5页World Chinese Medicine

基　　金：福建省教育厅中青年教师教育科研项目(JAT170287);福建省创双高重点专科项目(闽卫中医函2017-881)。

摘　　要：目的:研究丹参饮通过介导过氧化物酶体增殖物激活型受体γ(PPAR-γ)/肝X受体α(LXR-α)/三磷酸腺苷结合盒转运体A1(ABCA1)信号通路减轻大鼠冠状动脉粥样硬化的作用机制。方法:选取无特定病原体(SPF)级40只健康Wistar大鼠作为实验动物,随机分为空白组、冠状动脉粥样硬化组(对照组)、丹参饮低剂量观察组(低剂量组)和丹参饮高剂量观察组(高剂量组),比较各组大鼠的炎症介质水平、ICAM-1水平、脂代谢水平、PPAR-γ、LXR-α和ABCA1 mRNA表达和蛋白表达。结果:对照组、低剂量组和高剂量组的三酰甘油(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)均高于空白组(P<0.05),低剂量组和高剂量组的TG、TC和LDL-C均低于对照组(P<0.05),且高剂量组低于低剂量组(P<0.05),但4组的HDL-C表达差异无统计学意义(P>0.05);对照组、低剂量组和高剂量组的炎症介质和ICAM-1表达均高于空白组(P<0.05),低剂量组和高剂量组的炎症介质和ICAM-1表达均低于对照组(P<0.05),且高剂量组低于低剂量组(P<0.05);对照组、低剂量组和高剂量组的PPAR-γ、LXR-α和ABCA1 mRNA和蛋白表达均高于空白组(P<0.05),且低剂量组和高剂量组明显高于对照组(P<0.05),高剂量组明显高于低剂量组(P<0.05)。结论:丹参饮可以通过对PPAR-γ-LXR-α-ABCA1信号通路的介导,发挥出抗冠状动脉粥样硬化的作用。Objective:To study on Danshen Drink by mediating peroxisome proliferator-activated receptor γ(PPAR-γ)/liver X receptorα(LXR-α)/adenosine triphosphate binding cassette transporter A1(ABCA1)signaling pathway to alleviate the mechanism of coronary atherosclerosis of rats.Methods:A total of 40 healthy Wistar rats of SPF-free grade were selected as the experimental animals,and were randomly divided into a blank group,a coronary atherosclerosis group(control group),Danshen Drink low-dose treatment group(low-dose group)and Danshen Drink high Dose treatment group(high-dose group).The levels of inflammatory mediators,ICAM-1,lipid metabolism,PPAR-γ,LXR-αand ABCA1 mRNA expression and protein expression in each group of rats were compared.Results:The triacylglycerol(TG),cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)of the control group,low-dose group and high-dose group were all higher than those of the blank group(P<0.05),the TG,TC and LDL-C of the low-dose group and the high-dose group were lower than those of the control group(P<0.05),and the high-dose group was lower than the low-dose group(P<0.05),but there was no statistically significant difference in the HDL-C expression of 4 groups(P>0.05);the expressions of inflammatory mediators and ICAM-1 in the control group,low-dose group and high-dose group were higher than those in the blank group(P<0.05),low-dose group and high-dose group The expressions of inflammatory mediators and ICAM-1 in the control group were lower than those in the control group(P<0.05).The protein expressions of PPAR-γ、LXR-αand ABCA1 mRNA were higher than those of the blank group(P<0.05).And the low-dose and high-dose groups were significantly higher than those of the control group(P<0.05),and the high-dose group was significantly higher than the low-dose group(P<0.05).Conclusion:Danshen Drink can play an anti-coronary atherosclerosis effect by mediating the PPAR-γ-LXR-α-ABCA1 signaling pathway.

关 键 词：丹参饮 冠状动脉粥样硬化 过氧化物酶体增殖物激活型受体Γ 肝X受体Α 三磷酸腺苷结合盒转运体A1 丹参 檀香 砂仁 冠心病预防 

分 类 号：R289.5[医药卫生—方剂学] R541.4[医药卫生—中药学]