安肠愈疡汤对溃疡性结肠炎大鼠三叶因子3蛋白表达及肠道菌群的影响  

Effects of Anchang Yuyang Decoction on TFF3 Protein Expression and Intestinal Flora in Rats with Ulcerative Colitis

作　　者：王晗潞 王帅[2] 孙大娟[2] 梁峻尉[2] 尹德菲 迟莉丽[2] WANG Hanlu;WANG Shuai;SUN Dajuan;LIANG Junwei;YIN Defei;CHI Lili(College of Traditional Chinese Medicine,Shandong University of Chinese Medicine,Jinan 250014,China;Shandong Provincial Hospital of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学中医学院,山东济南250014 [2]山东省中医院,山东济南250014

出　　处：《中国中医药信息杂志》2021年第7期71-76,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81673969)。

摘　　要：目的观察安肠愈疡汤对溃疡性结肠炎大鼠三叶因子3(TFF3)蛋白表达及肠道菌群的影响。方法36只SD大鼠随机分为空白组、模型组、美沙拉嗪组和中药低、中、高剂量组,除空白组外,其余5组均自由饮用4.5%DSS溶液7 d制备溃疡性结肠炎大鼠模型,各给药组于造模第2日开始分别给予相应浓度药物干预,连续2周。干预结束后行结肠黏膜组织病理学评分,Western blot检测结肠组织TFF3蛋白表达,16S rDNA高通量测序分析粪便菌群。结果与空白组比较,模型组大鼠结肠组织病理学评分升高,TFF3蛋白表达下调,肠道菌群丰度、多样性降低,拟杆菌属丰度升高,乳杆菌属丰度下降;与模型组比较,各给药组结肠组织病理学评分降低,TFF3蛋白表达上调,肠道拟杆菌属丰度下降,乳杆菌属丰度升高,其中中药高剂量组差异最明显,中药中、高剂量组和美沙拉嗪组肠道菌群丰度、多样性升高。结论安肠愈疡汤可通过上调肠黏膜修复因子表达,恢复肠道菌群多样性,促进益生菌生长,抑制致病菌生长,进而修复肠黏膜。Objective To observe the effects of Anchang Yuyang Decoction on TFF3 protein and intestinal flora in rats with ulcerative colitis.Methods Totally 36 SD rats were randomly divided into blank group,model group,Anchang Yuyang Decoction low-,medium-,and high-dosage groups and mesalazine group.Except the blank group,the other 5 groups were given 4.5%DSS solution for modeling.Each administration group was given corresponding drug for intervention from the second day of modeling for 2 weeks.After the intervention,the colon mucosal histopathology score(HPS)was performed on each group;the TFF3 protein expression in colon tissue was measured by Western blot;the fecal flora was analyzed by 16S rDNA high-throughput sequencing.Results Compared with the blank group,the colon mucosal HPS score of the model group increased,the expression of TFF3 protein was down-regulated,the abundance and diversity of the intestinal flora decreased,and the abundance of Bacteroides increased,the abundance of Lactobacillus decreased.Compared with the model group,the colon mucosal HPS scores of each medication group decreased.The expression of TFF3 protein was up-regulated,the abundance of Bacteroides decreased,and the abundance of Lactobacillus increased,in which the difference in the Anchang Yuyang Decoction high-dosage group was the most obvious.The abundance and diversity of the intestinal flora in the Anchang Yuyang Decoction medium-and high-dosage groups and mesalazine group increased.Conclusion Anchang Yuyang Decoction can repair the intestinal mucosa by up-regulating the expression of intestinal mucosal repair factors,restore the diversity of intestinal flora,promote the growth of probiotics,and inhibit the growth of pathogenic bacteria.

关 键 词：安肠愈疡汤 溃疡性结肠炎 TFF3蛋白 肠道菌群 大鼠 

分 类 号：R285.5[医药卫生—中药学]