DBH基因多态性、碘氟及其交互作用与儿童智力的关联研究  

Association of Dopamine Beta-hydroxylase Gene Polymorphism,Iodide,Fluoride,and Their Interaction with Children's Intelligence

作　　者：郭百慧 虞静雯 崔玉山[2] 高童宁 赵亮[2] 魏文彬 刘洪亮 GUO Baihui;YU Jingwen;CUI Yushan;GAO Tongning;ZHAO Liang;WEI Wenbin;LIU Hongliang

机构地区：[1]天津医科大学公共卫生学院,300070 [2]天津市疾病预防控制中心 [3]天津市卫生健康委员会

出　　处：《环境卫生学杂志》2021年第2期134-140,共7页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家自然科学基金(81372934,81573107)。

摘　　要：目的 探讨多巴胺β羟化酶(dopamine beta-hydroxylase,DBH)基因多态性、碘氟及其交互作用对儿童智力影响。方法 在天津市根据历史水碘浓度选择3所学校,根据班级整群抽样方法选取299名儿童,采用《联合型瑞文测验》测定儿童智力水平,采集尿样后分别使用砷铈催化分光光度法和氟离子选择电极法测定尿碘及尿氟水平,采用聚合酶链式反应—限制性片段长度多态性方法检测DBH基因型。结果 高碘二区的(10~12)岁儿童智力(108.24±13.69)低于高碘一区(114.48±11.91)和适碘地区(113.10±12.54),且差异有统计学意义(F=3.45,P<0.05),多因素Logistic回归分析中只有尿氟是智力损伤的危险因素(OR=2.32,95%CI:1.09~4.95,P<0.05),未发现DBH基因与尿氟、尿碘对智力有交互作用,DBH基因在碘、氟导致智力损伤中无中介效应。结论 天津市水碘浓度>300 μg/L地区(10~12)岁儿童智力水平下降,未发现DBH基因与尿碘尿氟对智力有交互作用及中介效应,还需进一步研究。Objective To investigate the influence of dopamine beta-hydroxylase (DBH) gene polymorphism, iodine, fluoride, and their interaction on children's intelligence. Methods Three schools were selected according to the history of water iodine concentration in Tianjin, and then cluster sampling was performed to select 299 children. Combined Raven's Test was used to measure the children's intelligence level;urine samples were collected and then arsenic-cerium catalytic spectrophotometry and the fluoride-ion selective electrode method were used to measure urinary iodine and fluoride levels, respectively;polymerase chain reaction-restriction fragment length polymorphism was used to determine DBH genotype. Results The children aged (10-12) years in the high iodine area 2 had a significantly lower intelligence level than those in the high iodine area 1 and the suitable iodine area (108.24±13.69 vs 114.48±11.91/113.10±12.54, F=3.45, P<0.05). The multivariate logistic regression analysis showed that urinary fluoride was a risk factor for intelligence impairment (OR=2.32, 95%CI: 1.09-4.95, P<0.05). The DBH gene, urinary fluoride, and urinary iodine had no interaction with intelligence, and the DBH gene had no mediating effect on intelligence impairment induced by iodine and fluoride. Conclusion The intelligence level of children aged (10-12) years tends to decrease in the areas where the concentration of water iodine is over 300 μg/L in Tianjin. The DBH gene, urinary fluoride, and urinary iodine have no interaction with intelligence, and the DBH gene has no mediating effect on intelligence, which requires further research.

关 键 词：智力 尿氟 尿碘 环境 基因多态性 交互作用 

分 类 号：R12[医药卫生—环境卫生学]