倒木木材腐朽真菌高产纤维素酶培养条件优化  

Optimization of culture conditions for high-yield cellulase of fallen wood and wood-decay fungi

作　　者：陈妍 简立燕 陈凤 李宁 王李睿 何东进[1,2] CHEN Yan;JIAN Liyan;CHEN Feng;LI Ning;WANG Lirui;HE Dongjin(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Southern Forest Resources and Environmental Engineering Technology Research Center,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省南方森林资源与环境工程技术研究中心,福建福州350002

出　　处：《森林与环境学报》2021年第4期382-387,共6页Journal of Forest and Environment

基　　金：国家自然科学基金项目(31370624);福建农林大学科技创新项目(KF2015032;KF2015033)。

摘　　要：为提高纤维素的降解效率、降低能耗、减少污染,加快长苞铁杉倒木分解,促进其自然更新,以天宝岩国家级自然保护区内长苞铁杉倒木中的郝克(氏)青霉菌(CB1)、小刺青霉菌(CB2)、光孢青霉菌(CB3)3株木材腐朽真菌菌株为对象,利用单因素试验和正交试验的方法,对培养基的碳源、氮源、初始pH值、摇床转速、接种量、培养温度进行分析,研究其最佳产酶条件。结果表明:以稻草粉+麦麸为复合碳源、以硝酸钾为氮源、初始pH值5、摇床转速140 r·min^(-1)、培养温度28℃时,3株青霉菌产酶量均达最高值;CB1和CB3在接种量(体积分数)7%时,产酶量达最高值,CB2在接种量(体积分数)5%时,产酶量最高。通过正交试验得出,CB1和CB3的最佳产酶条件均为:初始pH值5,摇床速度140 r·min^(-1),接种量(体积分数)7%,培养温度28℃;CB2的最佳产酶条件为:初始pH值5,摇床速度140 r·min^(-1),接种量(体积分数)5%,培养温度28℃,三者均比优化前(CK)的单因素最佳产酶量有所提高。To accelerate the decomposition of fallen hemlock Tsuga longibracteata and promote its natural regeneration,three wood-decaying fungi strains(Penicillium harkeli,CB1;P.parvum,CB2;and P.photosporum,CB3)in fallen trees(Tsuga longibracteata)in Tianbaoyan National Nature Reserve were studied.Single factor and orthogonal experiments were used to analyze carbon source,nitrogen source,initial pH value,shaker speed,inoculation amount,and culture temperature.The optimal enzyme production conditions were also studied.Enzyme production of the three strains was greatest when rice straw powder and wheat bran were used as the composite carbon source,potassium nitrate as the nitrogen source,initial pH was 5,shaker speed was 140 r·min^(-1),and culture temperature was 28℃.The enzyme production was greatest when the inoculum was 7%(v/v)for CB1 and CB3,and 5%(v/v)for CB2.The orthogonal experiment revealed that the best enzyme production conditions for CB1 and CB3 were as follows:initial pH value of 5,shaker speed of 140 r·min^(-1),7%(v/v)inoculum,and culture temperature of 28℃.The optimal conditions for CB2 were as follows:initial pH value of 5,shaker speed of 140 r·min^(-1),5%(v/v)inoculum,and culture temperature of 28℃.The optimized production levels exceeded the single factor enzyme production before optimization.

关 键 词：长苞铁杉 纤维素酶 木材腐朽菌 培养条件优化 

分 类 号：S718.81[农业科学—林学]