基于纳米磁流体尸体CT血管造影的可行性探究  

Research on the feasibility of applying magnetic nanoparticle(MNP)ferrofluid to postmortem CT angiography(PMCTA)

作　　者：夏志远 Christine Bruguier Fabrice Dedoui Silke Grabherr Marc Augsburger 刘蓓蓓 Xia Zhiyuan;Christine Bruguier;Fabrice Dedoui;Silke Grabherr;Marc Augs burger;Liu Beibei(Dian Research Center for Postmortem Imaging&Angiography,Zhejiang Dian Institute of Forensic Sciences,Hangzhou 310000,China;University Center of Legal Medicine,Lausanne-Geneva(CURML),Lausanne CHI 000,Switzerland;Service de Medecine Legale,Hopital de Rangueil,Toulouse 50032,France)

机构地区：[1]浙江迪安鉴定科学研究院迪安鉴定虚拟解剖研究中心,浙江杭州310000 [2]洛桑和日内瓦大学法医中心,瑞士洛桑CH1000 [3]兰格勒医院法医学服务中心,法国图卢兹50032

出　　处：《中国法医学杂志》2021年第3期230-233,共4页Chinese Journal of Forensic Medicine

基　　金：国家留学基金(201707070113)。

摘　　要：目的初步探索纳米磁流体(magnetic nanoparticle ferrofluid,MNPF)用于尸体计算机断层扫描血管造影(postmortem computed tomography angiography,PMCTA)的可行性。方法纳米粒子γ-Fe2O3(30nm)通过油酸(oleic acid,OA)进行表面修饰,修饰后物质移至液体石蜡油(paraffin oil,PO)中,使用高速搅拌器搅拌,制备成γ-Fe2O3含量为200g/L的MNPF。使用手动负压灌注法,将MNPF、PO和OA分别导入3只牛心脏的冠状动脉及左心室。将灌有不同液体的牛心移至CT机上扫描,观察冠脉或左心室显影情况。结果灌注MNPF的牛心,其冠脉呈现高信号,3D重建后可以较好地显示冠脉解剖形态。灌注PO和OA的牛心,冠脉呈现低信号,无法通过3D重建显示冠脉解剖形态。结论MNPF可以提高冠脉显影效果,对于PMCTA可能有一定的研究价值。Objective To study the feasibility of applying magnetic nanoparticle ferrofluid(MNPF)to postmortem computed tomography angiography(PMCTA).Methods Surface of nanoparticleγ-Fe2O3(30 nm)was modified by oleic acid(OA).The modified material was transferred into paraffin oil(PO)and mixed by a high power mixer to prepare MNPF.MNPF,PO and OA were introduced into corontary artery and left vetricle of three bovine hearts by manual negativepressure perfusion method.Results A hyperintensity of coronary artery was observed in the bovine heart perfused with MNPF,and the radiological morphology of its coronary artery could be visualized on 3 D reconstruction images.On the contrary,hypointensities were observed in the bovine hearts perfused with PO and OA,and the coronary arteries were not visualized on 3 D reconstruction images.Conclusion MNPF can enhance the contrast of coronary artery,which may have certain research value for PMCTA.

关 键 词：纳米磁流体 尸体计算机断层扫描血管造影 Γ-FE2O3 油酸 石蜡油 

分 类 号：DF795.1[医药卫生—法医学]