单分子测序技术法医学应用研究进展  

Research progress of single molecule sequencing in forensic medicine

作　　者：杨春 吴文雪 于小淇 司晗 纪娜 陶陆阳[1,2] 张明阳[1,2] Yang Chun;Wu Wenxue;Yu Xiaoqi;Si Han;Ji Na;Tao Luyang;Zhang Mingyang(Department of Forensic Medicine,Soochow University,Suzhou 215123,China;Institute of Forensic medicine,Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学医学部法医学系,江苏苏州215123 [2]苏州大学司法鉴定中心,江苏苏州215123

出　　处：《中国法医学杂志》2021年第3期302-305,309,共5页Chinese Journal of Forensic Medicine

基　　金：国家自然基金资助项目(82071382);江苏高校优势学科建设工程资助项目(PAPD);江苏省大学生创新创业训练项目资助(202010285139Y);苏州市科技发展计划项目(SYS2020089);苏州大学医学部学生课外科研项目(2020YXBKWKY020)。

摘　　要：测序技术从20世纪中后期开始出现,取得了飞速发展的同时,并逐渐应用于多个领域。单分子测序技术作为第三代测序技术,可以说是测序技术历史性的一次革新。单分子测序技术主要通过实时动态监测核苷酸序列变化在分子水平进行测序分析,具有超长读长、测序速度快、RNA序列和表观修饰位点直接检测等优点,为科研人员开展试验提供丰富的选择。本文系统地介绍了单分子测序技术的概况,并对其在法医学领域的运用进行展望。Sequencing technologies appeared in the middle and late 20 th century and were applied in various kinds of fields.In the past 10 years,DNA sequencing technologies have been developing rapidly and single molecule sequencing technology is a historic innovation among them.Single molecule sequencing reads nucleotide sequences at the molecular level for real-time sequencing.It has the advantages of long reading,fast sequencing,direct detection of RNA sequence and epigenetic modifications,which provides a new option for researchers.This article systematically introduces the general situation of single-molecule sequencing technology and its application in the field of forensic medicine.

关 键 词：单分子测序技术 法医学 法医病理学 法医临床学 法医物证学 

分 类 号：DF795.1[医药卫生—法医学]