超声辅助酶法提取金银花多糖的工艺优化  

Process Optimization of Ultrasonic-assisted Aqueous Enzymatic Method in the Extraction of Lonicera japonica Polysaccharides

作　　者：吕欣 谭春玲 麦名娜 林容芳 张美霞 LYU Xin;TAN Chun-ling;MAI Ming-na;LINRong-fang;ZHANG Mei-xia(College of Forestry and Life Science,Chongqing University of Arts and Sciences,Chongqing 402160,PRC)

机构地区：[1]重庆文理学院林学与生命科学学院,重庆402160

出　　处：《湖南农业科学》2021年第4期90-95,共6页Hunan Agricultural Sciences

基　　金：重庆市大学生创新创业训练项目(201810642009)。

摘　　要：以金银花为原材料,采用超声辅助酶法提取金银花中的多糖,在单因素试验的基础上,选用酶用量、超声温度、料液比为自变量,以多糖得率为响应值,通过响应面Box-Behnken优化金银花多糖的提取工艺。结果表明:超声辅助酶法提取金银花多糖的最佳工艺为料液比1∶48(g/mL)、超声温度50.80℃、酶用量2.56%、超声时间60 min、超声功率200 W、酶解pH值为5,在此条件下金银花多糖平均得率为54.275%。Taking Lonicera japonica as raw material,Lonicera japonica polysaccharides were extracted by ultrasonic-assisted aqueous enzymatic method.On the basis of single factor experiment,the extraction process of Lonicera japonica polysaccharides was optimized by response surface Box-Behnken design using the parameters of enzyme dosage,ultrasound temperature and solid-liquid ratio as independent variables and the yield of polysaccharides as response value.The optimal extraction process of Lonicera japonica polysaccharides by ultrasonic-assisted aqueous enzymatic method was obtained:solid-liquid ratio 1∶48(g/mL),ultrasound temperature 50.80℃,enzyme dosage2.56%,ultrasound treatment 60 min,ultrasound power 200 W,and enzymolysis pH 5.Under these conditions,the average yield of Lonicera japonica polysaccharides was 54.275%.

关 键 词：金银花 多糖提取 超声辅助酶法 响应面 工艺优化 

分 类 号：R284.2[医药卫生—中药学] TS209[医药卫生—中医学]