不同产地高节竹竹叶次生代谢物与土壤微生物的关系分析  

Secondary Metabolites of Bamboo Leaves from Phyllostachys prominens and Their Relationship with Soil Microorganism in Different Producing Areas

作　　者：徐小博 王选年[1] 徐萍 岳永德 汤锋 王进 姚曦 Xu Xiaobo;Wang Xuannian;Xu Ping;Yue Yongde;Tang Feng;Wang Jin;Yao Xi(School of Life Sciences and Basic Medicine,Xinxiang University,Xinxiang Henan 453003,China;International Centre for Bamboo and Rattan,Beijing 100102,China)

机构地区：[1]新乡学院生命科学与基础医学学院,河南新乡453003 [2]国际竹藤中心,北京100102

出　　处：《西南林业大学学报（自然科学）》2021年第4期18-24,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：“十三五”国家重点研发计划课题(2016YFD0600801)资助;2020年度新乡市软科学研究计划项目(RKX2020015)资助;河南省高等学校重点科研项目(20A180025)资助。

摘　　要：为研究不同产地高节竹竹叶次生代谢物差异、土壤微生物特征及两者之间关系,测定不同产地竹叶中9种次生代谢产物含量和土壤中3种微生物数量、微生物量碳、微生物量氮及3种土壤酶活性等指标,利用SPSS软件对土壤微生物特征与竹叶次生代谢产物含量进行相关性分析。结果表明:不同产地高节竹竹叶的9种次生代谢产物差异显著,不同产地的3种土壤微生物、土壤酶活和微生物量碳、微生物量氮等土壤微生物特征有明显差异。高节竹竹林土壤细菌数量与竹叶荭草苷、对香豆酸和牡荆苷含量呈正相关关系,土壤真菌数量与竹叶异荭草苷和木犀草苷含量呈显著正相关关系;高节竹竹叶次生代谢相关产物的含量与土壤中3种酶活性的相关性不显著。因此,土壤微生物特征能够影响高节竹竹叶次生代谢产物的积累,但是不同次生代谢产物对土壤微生物特征的响应不同,增加土壤细菌可以提高高节竹竹叶荭草苷、对香豆酸和牡荆苷含量,增加土壤真菌可以提高高节竹竹叶异荭草苷和木犀草苷。In order to clarify the differences in secondary metabolites in Phyllostachys prominens leaves from different habitats and their relationship with soil properties,the contents of 9 secondary metabolites,3 kinds of soil microorganisms,SMBC,SMBN and 3 soil enzyme activities were determined.Then the correlation between soil microbial characteristics and secondary metabolites was analyzed by SPSS software.Results showed there were significant differences in the 9 secondary metabolites of P.prominens leaves from different producing areas.Three soil microorganisms,soil enzyme activities,microbial biomass carbon,and microbial biomass nitrogenother of different production areas were significantly different.There was a positive correlation between the amount of soil bacteria in bamboo forest and orientin,p-Coumaric acid,vitexin.There was a significant positive correlation between the soil fungi quantity and the content of isoorientin and luteolin in P.prominens leaves.There was no significant correlation between the content of secondary metabolism-related products of P.prominens leaves and the activities of 3 enzymes in the soil.In summary,soil microbial characteristics can affect the accumulation of secondary metabolites of P.prominens leaves,but different secondary metabolites respond differently to soil microbial characteristics.Increasing soil bacteria can increase the content of orientin,p-Coumaric acid,and vitex-in.Increasing soil fungi can increase isoorientin and luteolin.

关 键 词：高节竹 次生代谢物 土壤微生物 土壤酶活性 

分 类 号：S713[农业科学—林学]