2个蝴蝶兰品种对低温胁迫的生理响应  

Physiological Response of Cold Stress on 2 Cultivars of Phalaenopsis amabilis

作　　者：马晓华[1] 胡青荻 张庆良[1] 钱仁卷[1] 刘洪见[1] 张旭乐[1] Ma Xiaohua;Hu Qingdi;Zhang Qingliang;Qian Renjuan;Liu Hongjian;Zhang Xule(Institute of Subtropical Crops of Zhengjiang Province,Wenzhou Zhejiang 325005,China)

机构地区：[1]浙江省亚热带作物研究所,浙江温州325005

出　　处：《西南林业大学学报（自然科学）》2021年第4期72-78,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：温州市种子种苗科技创新专项项目(Z20170007)资助。

摘　　要：以2年生‘绿熊’和‘安娜’蝴蝶兰品种为试验材料,测定其在低温(5℃)下处理12、24、48 h后叶片净光合速率、气孔导度、相对电导率、脯氨酸、可溶性蛋白、丙二醛等的含量及超氧化物歧化酶、过氧化氢酶、过氧化物酶活性,分析低温胁迫对不同蝴蝶兰品种叶片表观症状、光合特性、抗氧化酶活性及渗透调节物质含量的影响。结果表明:5℃低温胁迫显著降低了‘绿熊’和‘安娜’的净光合速率、气孔导度,并增加了丙二醛、脯氨酸、可溶性糖等的含量,相对电导率值及超氧化物歧化酶、过氧化氢酶、过氧化物酶酶活性,并随着处理时间的增加,其变化程度逐渐加深,但‘绿熊’净光合速率降低幅度低于‘安娜’,且其叶片形态、可溶性糖含量、可溶性蛋白含量显著高于‘安娜’。由于在5℃低温条件下,‘绿熊’叶片的表观症状良好,且净光合速率、气孔导度、过氧化氢酶活性、可溶性蛋白含量和可溶性糖含量等显著高于‘安娜’,表明‘绿熊’的抗冷性优于‘安娜’。Two-year-old Phalaenopsis amabilis‘Green bear’and P.amabilis‘Anna’were used as experimental materials to determine the net photosynthetic rate(Pn),stomatal conductance(Gs),relative electrical conductivity(REC),proline,soluble protein and malondialdehyde(MDA)contents and superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)activities at 5℃for 12,24 and 48 hours.The effects of cold stress on leaf morphology,photosynthetic parameters,antioxidant enzyme activity and content of osmotic adjustment substance in different cultivars of P.amabilis were analyzed.The results showed that 5℃stress significantly reduced the Pn and Gs of P.amabilis‘Green bear’and P.amabilis‘Anna’,and increased the contents of MDA,proline and soluble sugar,as well as SOD,CAT,POD enzyme activities and REC.And with the increase of the treatment time,the change degree deepens gradually.However,the decrease in Pn of P.amabilis‘Green bear’was lower than that of P.amabilis‘Anna’,and its leaf morphology,soluble sugar and soluble protein content were significantly higher than that of P.amabilis‘Anna’.Under 5℃cold stress,the leaf morphology of P.amabilis‘Green bear’was better,Pn,Gs,CAT activity,content of soluble protein and soluble sugar were significantly higher than that of P.amabilis‘Anna’.This research proves the cold resistance of P.amabilis‘Green bear’better than that of P.amabilis‘Anna’.

关 键 词：蝴蝶兰 低温 光合作用 渗透调节 保护酶 

分 类 号：S332[农业科学—作物遗传育种]