接种平滑白蛋巢菌对蒿柳根系分泌物代谢组的影响  

Effects of Crucibulum laeve Inoculation on Metabolome in Root Exudate from Salix viminalis L.

作　　者：马晓东 李霞 邹竣竹 白媛媛 孙振元[1] 韩蕾[1] MA Xiao-dong;LI Xia;ZOU Jun-zhu;BAI Yuan-yuan;SUN Zhen-yuan;HAN Lei(Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;College of Agriculture and Bioengineering(Peony Institute),Heze University,Heze 274000,Shandong,China)

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [2]菏泽学院农业与生物工程学院(牡丹学院),山东菏泽274000

出　　处：《林业科学研究》2021年第3期46-55,共10页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金“雄安新区水体净化灌木柳引选与应用模式”(CAFYBB2018ZB002)。

摘　　要：[目的]虽然已有研究表明植物-白腐真菌联合修复是一种更高效的多环芳烃(PAHs)污染土壤修复策略,但目前尚不清楚接种白腐真菌对植物根系分泌物的影响,后者与根际PAHs降解菌的生长和PAHs的生物降解密切相关。基于此,我们开展PAHs污染土壤中接种白腐真菌对植物根际土壤代谢组的影响研究。[方法]在温室中设置了PAHs污染土壤的盆栽修复实验,以蒿柳(Salix viminalis L.)作为植物修复树种,基于LC-MS检测,重点比较PAHs污染土壤中接种平滑白蛋巢菌(Crucibulum laeve)对蒿柳根际土壤代谢谱的影响。[结果]分别在正离子模式(POS)和(NEG)模式下鉴定出881和828种代谢物,不同处理之间代谢物的组分及其含量存在显著差异。比较分析了差异代谢物并鉴定出18个潜在根系分泌物组分,接种平滑白蛋巢菌显著减少了蒿柳根际土壤代谢物的组分和含量,其中16种根系分泌物的含量明显降低。[结论]接种白腐真菌促进了植物根系对广谱土壤化合物的吸收能力,推测植物根系对白腐真菌的刺激作用做出应答,引发了植物PAHs提取能力提高,对揭示植物-白腐真菌联合修复的作用机理有重要意义。[Objective]To study the effects of plant/white rot fungi(WRF)inoculation on rhizosphere soil metabolome in root exudate of plants cultivated in PAH-contaminated soil.[Method]A pot experiment was conducted in greenhouse for bioremediation of PAH-contaminated soils and Salix viminalis L.was used as phytoremediation materials.Based on liquid chromatography-mass spectrometry(LC-MS)and metabolomics method,the test was focused on comparing the effect of inoculating Crucibulum laeve on metabolic profiling of rhizosphere PAH-contaminated soil of S.viminalis.[Result]Under POS and NEG mode,881 and 823 compounds were detected and identified in metabolic profiling,respectively.Among them,18 compounds were identified and assumed to be potential root exudates components.The component variety and content of rhizosphere metabolites were remarkably reduced by C.laeve inoculating,which including 16 root exudate components.[Conclusion]In this study,it is showed that inoculation with WRF can promote the uptake capability of roots to a broad spectrum of soil compounds.It is speculated that the response of plant roots to WRF stimulating leads to the improvement of PAHs phytoextraction capacity.This is of great significance for revealing the mechanism of plant/WRF combination remediation of PAH-contaminated soils.

关 键 词：蒿柳 PAHS污染 平滑白蛋巢菌 根际土壤 代谢组 

分 类 号：S718.43[农业科学—林学]