欧美杨细菌性溃疡病菌的寄主范围调查及其寄主抗性物质的初步分析  

Investigation on Host Range, Host Resistance Substances of Bacterial Bark Canker of Populus×euramericana

作　　者：李永[1] 常聚普 郭利民 孙尚 杨旭琦 朴春根[1] LI Yong;CHANG Ju-pu;GUO Li-min;SUN Shang;YANG Xu-qi;PIAO Chun-gen(Research Institute of Forest Ecology Environment and Protection,Chinese Academy of Forestry,Beijing 100091;Puyang Academy of Forestry,Puyang 457000)

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,北京100091 [2]濮阳市林科所,河南濮阳457000

出　　处：《林业科学研究》2021年第3期56-63,共8页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017QC004)。

摘　　要：[目的]为明确欧美杨细菌性溃疡病菌的寄主范围、欧美杨抗性品种及其可能的抗性活性物质。[方法]通过田间接种方法开展欧美杨细菌性溃疡病菌的寄主范围、杨树品系抗性调查,并利用高效液相色谱分析杨树抗感品种树皮中活性物质含量及其染病前后含量变化情况,同时利用抑菌实验验证潜在抗性物质的活性。[结果]研究发现欧美杨细菌性溃疡病主要危害黑杨派美洲黑杨、欧美杂交杨的品系以及旱柳,而欧洲黑杨品系具有高度抗菌能力;本研究的30个美洲黑杨和欧美杂交杨品系中,中林46杨、2025杨、2001杨、313杨、中荷1号杨和16-09杨是高感病品系;16-10杨、豫抗1号杨为高抗病品系,而131杨、03-59杨、02-212杨和03-34杨为抗病杨树品种;杂交柳和垂柳抗病,金丝柳轻微感病;栎属和青杨派的种类和品系具有高度抗性。高效液相色谱分析显示,抗病和高感病品系树皮中的邻苯二酚、苯甲酸、绿原酸、儿茶素的含量较高,其中邻苯二酚和儿茶素在病菌侵染时含量明显增加。[结论]明确了欧美杨细菌性溃疡病的主要危害对象和寄主范围,明确了具有抗性、感病的杨树品系,证明了邻苯二酚和儿茶素与杨树抗病性密切相关,该研究结果为欧美杨的栽培和病害防治提供技术支持和防治建议,该研究结果对我国欧美速生杨的细菌性溃疡病的防治具有重要意义。[Objective]This Research is to investigate the host range of bacterial canker of Populus×euramericana,the resistant varieties and its possible resistant substances. [Method]The host range of the pathogen and the resistant varieties were determined by field investigation and inoculation tests. The content of active substances in the bark of resistant and susceptible varieties was analyzed by HPLC. [Result]The host of bacterial pathogen of Populus ×euramericana canker are Populus deltoides, Populus×euramericana and Salix matsudana. Zhonglin 46, 2025, 2001,313, Zhonghe-1 and 16-09 were highly susceptible to the bark canker disease;Yukang 1 and 16-10 were highly resistant varieties, 131, 03-59, 02-212 and 03-34 were resistant varieties. Hybrid willows and Salix babylonica are resistant to the pathogen, and Salix × aureo-pendula can be affected, but do not cause serious disease. The HPLC analysis showed that the contents of catechol, benzoic acid and chlorogenic acid in the bark of resistant and highly susceptible poplar varieties were significantly different. The catechol and catechin increased when the hosts were infected by L.populi. [Conclusion]In this study, the host range of bacterial canker of Populus × euramericana were determined,and resistant and highly susceptible poplar varieties were screened, and the catechol and catechin are important compound for host to defense the pathogen infection. The results will be helpful to the control of the bacterial disease of poplar.

关 键 词：欧美杨 溃疡病 抗性物质 细菌 

分 类 号：S763.13[农业科学—森林保护学]