毛竹释放挥发物对空气负离子及微生物的影响  

Effects of volatile components of Phyllostachys edulis on air negative ion and microorganisms

作　　者：吕嘉欣 王翔[2] 项亨旺 韦赛君 王彬[1] 高岩[1] 张汝民[1] LV Jia-xin;WANG Xiang;XIANG Heng-wang;WEI Sai-jun;WANG Bin;GAO Yan;ZHANG Ru-min(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Zhejiang Forest Resources Monitoring Center,Hangzhou 310020,Zhejiang,China;Hengwang Eco-tourism Development Co.LTD,Deqing 313200,Zhejiang,China)

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室,浙江杭州311300 [2]浙江省森林资源监测中心,浙江杭州310020 [3]亨旺生态旅游发展有限公司,浙江德清313200

出　　处：《竹子学报》2020年第3期49-57,共9页Journal of Bamboo Research

基　　金：浙江省省院合作项目(2018SY07);国家自然科学基金项目(31470704)。

摘　　要：为了研究毛竹释放的挥发性有机化合物(VOCs)对空气微生物的影响,研究采用动态顶空气体循环吸附法和热脱附-气相色谱-质谱(TDS-GC-MS)联用技术,分析了近自然状态下毛竹叶片释放的VOCs和不同毛竹林内空气VOCs的组分与含量;测定了林内空气负离子及微生物浓度,并进行微生物单体试验来验证挥发物对微生物的抑制作用。结果显示:毛竹单株共检测出挥发物35种,主要物质有醛类(26.2%)、酯类(21.9%)、醇类(18.4%)和萜烯类(16.6%);不同季节毛竹林内VOCs种类和相对含量存在差异,主要表现为:夏>春>秋,春、夏季的醛类分别是秋季的30.3倍和39.8倍;春、夏、秋季的毛竹林内空气负离子浓度分别为1030、1975、547个·cm^(-3);毛竹林中空气微生物的浓度同林缘相比均有明显的降低,春、夏、秋季对细菌抑制率分别为72.4%、61.3%和78.4%(P<0.05),对真菌抑制率为62.9%、59.7%和72%(P<0.05),对放线菌抑制率为:61.9%、58%和72.1%(P<0.05);VOCs单体抑菌试验表明罗勒烯、癸醛、乙酸、叶醇对微生物的抑制作用显著。毛竹释放的VOCs对NAI的形成具有促进作用,对空气微生物生长具有抑制作用。In order to study the effects of of volatile organic compounds(VOCs)of Phyllostachys edulis on air microorganism,by using the dynamic systemic adsorption method and hot air stripping gas chromatography-mass spectrometry(TDS-GC-MS),the near natural Ph.edulis leaf VOCs,the composition and content of the VOCs in the Ph.edulis forest under near natural condition in different seasons,the concentrations of negative air ions and microorganisms in Ph.edulis forest were measured.The inhibition effect of volatiles on microorganisms was verified by microbe monomer test.The results showed that a total of 35 kinds of volatile substances were detected from a individual bamboo,including aldehydes(26.2%),esters(21.9%),alcohols(18.4%),terpenes(16.6%),etc.Thetypes and relative contents of VOCs of Ph.edulis forest were different between seasons,summer>spring>autumn.The aldehydes in spring and summer were 30.3 times and 39.8 times higher than that in autumn respectively.In spring,summer and autumn,the air anion concentration in the Ph.edulis grove was 1030,1975 and 547 per cubic centimeter respectively.The number of air microorganisms in the Ph.edulis varied dynamically in different seasons,and the concentration decreased significantly compared with the forest margin.The inhibition rates of bacteria in spring,summer and autumn were 72.4%,61.3% and 78.4%,respectively.The inhibition rate of fungi was 62.9%,59.7% and 72%.The inhibition rate of actinomycetes was 61.9%,58% and 72.1%.VOCs released by Ph.edulis can inhibit the growth of air microorganisms and promote the formation of NAI.

关 键 词：毛竹 植物挥发物 空气负离子 微生物 GC-MS 抑菌作用 

分 类 号：S795.7[农业科学—林木遗传育种]