狭小空间法构建抑郁症大鼠模型的优劣探索  

The Advantages and disadvantages of constructing depression rat model with narrow space method

作　　者：闫雪[1] 曾德浩[1] 李超群 张毅靖 祝赫 唐洪梅[1] YAN Xue;ZENG De-hao;LI Chao-qun;ZHANG Yi-jing;ZHU He;TANG Hong-mei(Depatment of Pharmacy,the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China;不详)

机构地区：[1]广州中医药大学第一附属医院药学部,广东广州510405 [2]南方医科大学中医药学院,广东广州510515

出　　处：《广东医学》2021年第5期534-539,共6页Guangdong Medical Journal

基　　金：广东省中医药局科研项目(20191121)。

摘　　要：目的采用狭小空间法构建抑郁症大鼠模型(M),检测抑郁症大鼠的生理指标、行为变化及海马区的改变;将其与常规抑郁模型——慢性温和不预知应激(CUMS)抑郁症大鼠的相应指标进行差异性比较;亟望为抑郁症大鼠模型构建提供一种新方法。方法取30只大鼠将其随机分成3组:(1)M模型抑郁症大鼠(M组):采用10 cm×10 cm×5 cm的孤笼构建成狭小空间,常规饲养,连续刺激4周。(2)CUMS模型抑郁症大鼠(CUMS组):将其给予禁食24 h/次、禁水24 h/次、鼠笼倾斜30°过夜/次、潮湿饲养过夜/次、4℃冰水游泳5 min/次、昼夜颠倒/次、夹尾1 min/次等刺激方式,应用随机数字表法决定每天采纳的2种刺激方式,刺激4周。(3)正常对照组:常规喂养4周。对3组大鼠的行为学变化进行比较,其中包括体重、饮水进食量、糖水偏好度和旷场实验活动行为情况;HE染色观察3组模型大鼠的海马组织形态学变化;ELISA方法检测海马组织中单胺类递质5-羟色胺(5-HT)的含量变化。结果M组与正常对照组相比,14 d后大鼠的体重、糖水偏好度差异有统计学意义(P<0.05);7 d后大鼠的饮水量、进食量、旷场试验的直立次数和方格间穿越次数差异均有统计学意义(P<0.05)。与CUMS组比较,M组14、21 d的体重差异有统计学意义(P<0.05,P<0.01);旷场试验的14、21 d直立次数和方格间穿越次数、28 d的方格间穿越次数均减少,差异有统计学意义(P<0.01),同时28 d的直立次数明显减少(P<0.05)。与正常对照组相比,M组、CUMS组海马组织神经元细胞数目较少。与正常对照组比较,M组和CUMS组大鼠海马组织5-HT水平显著降低(P<0.01),且M组与CUMS组之间比较差异均无统计学意义(P>0.05)。结论成功构建狭小空间抑郁症大鼠模型,该模型可能缩短抑郁症模型的成模时间。狭小空间抑郁症大鼠模型造模方法简便、稳定、省时,并且造模效果与慢性温和不预知应激抑郁症大鼠�Objective The depression rat model(M)was established using narrow space method;and the physiological indexes,behavioral changes and hippocampal changes of the depressed rats were assessed.These indicators were compared between the established model and the conventional depression model-chronic unpredictable mild stress(CUMS)depression rats.So thus to provide a new method for the construction of rat model of depression.Methods Thirty rats were randomly divided into 3 groups,including Group M,Group CUMS,and Group Control.In Group M,rats were kept in solitary cage of 10 cm*10 cm*5 cm,which simulated narrow space,for 4 weeks.In Group CUMS,rats were individually placed in acrylic cages for 4 weeks to induce social isolation;at the same time,4 cycles of weekly stress regimes were administered,consisting of the following stressors:30°cage tilt(overnight),food deprivation(24 h),cold swimming(5-min at 4℃),tail pinch(1-min),moist bedding(8 h)and overnight stroboscopic lighting.The stressors were administered daily in a random order for 4 consecutive weeks.In Group Control,rats were routinely raised for 4 weeks.The behavioral changes were compared,including body mass,water intake,sugar water preference test,open-field test.HE staining was used to observe the morphological changes of hippocampus.The content of 5-hydroxytryptamine(5-HT)in the hippocampus was assessed by ELISA.Results Compared with Group Control,the body mass and sugar water preference in the Group M were significantly different after 14 days(P<0.05);and the water intake,food intake,upright times and crossing times between squares in open field test were significantly different after 7 days(P<0.05).There was significant difference in the body mass between Group M and CUMS after 14 days(P<0.05),and the body mass of 21 days was also significantly different(P<0.01).Between Group M and CUMS,in the open-field test,the upright times,crossings on Day 14 and 21,and crossings time and upright times on Day 28 were significantly reduced in Group M(P<0.01).The numbers

关 键 词：抑郁症模型 行为学测评 海马 5-羟色胺 

分 类 号：R395.4[哲学宗教—心理学] R332[医药卫生—医学心理学]