The transcription factor FgNsf1 regulates fungal development,virulence and stress responses in Fusarium graminearum  

作　　者：SHI Dong-ya REN Wei-chao WANG Jin ZHANG Jie Jane Ifunanya MBADIANYA MAO Xue-wei CHEN Chang-jun 

机构地区：[1]College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,P.R.China [2]College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第8期2156-2169,共14页农业科学学报（英文版）

基　　金：This work was supported by the National Key Research&Development Program of China(2016YED0201007,2018YFD0201201 and 2018YFD0201000);the National Natural Science Foundation of China(31672065);the Agricultural Science and Technology Projects of Jiangsu Province,China(BE2018378,BA2018039,PZCZ201715,CX(19)3003,and CX(18)2005);the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX18_0670).

摘　　要：Nutrient and stress factor 1(Nsf1),a transcription factor containing the classical Cys2-His2(C_(2)H_(2))zinc finger motif,is expressed under non-fermentable carbon conditions and in response to salt stress in Saccharomyces cerevisiae.However,the role of Nsf1 in filamentous fungi is not well understood.In this study,the orthologue of Nsf1 was investigated in Fusarium graminearum(named FgNsf1),a causal agent of Fusarium head blight(FHB).The functions of FgNsf1 were evaluated by constructing a FgNSF1 deletion mutant,designated asΔFgNsf1,and its functional complementation mutantΔFgNsf1-C.Gene deletion experiments showed that the mycelial growth rate,asexual and sexual reproduction ofΔFgNsf1 were significantly reduced,but the pigment production ofΔFgNsf1 was remarkably increased compared with the PH-1 andΔFgNsf1-C.In addition,the tolerance ofΔFgNsf1 to osmotic pressures,cell wall-damaging agents and oxidative stress increased significantly.Sensitivity tests to different fungicides revealed thatΔFgNsf1 exhibited increased sensitivity to carbendazim(MBC)and tebuconazole,and enhanced tolerance to fludioxonil and iprodione than PH-1 andΔFgNsf1-C.The virulence ofΔFgNsf1 to wheat coleoptiles and flowering wheat heads were dramatically decreased,which was consistent with the decrease in the yield of deoxynivalenol(DON).All of these defects were restored by target gene complementation.These results indicated that FgNsf1 plays a crucial role in vegetative growth,asexual and sexual reproduction,stress responses,fungicide sensitivity,and full virulence in F.graminearum.

关 键 词：Fusarium graminearum nutrient and stress factor 1 stress responses VIRULENCE 

分 类 号：S432.44[农业科学—植物病理学]