Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera:Coccinellidae)  

作　　者：QU Cheng WANG Ran CHE Wu-nan LI Feng-qi ZHAO Hai-peng WEI Yi-yun LUO Chen XUE Ming 

机构地区：[1]College of Plant Protection,Shandong Agricultural University,Tai’an 271018,P.R.China [2]Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,P.R.China [3]Department of Pesticide Sciences,Shenyang Agricultural University,Shenyang 110866,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第8期2204-2213,共10页农业科学学报（英文版）

基　　金：This work was supported by the National Key Research and Develop Program of China(2017YFD0200400);the Shandong Province Modern Agricultural Technology System Peanut Innovation Team,China(SDAIT-04-08);the Beijing Leafy Vegetables Innovation Team of Modern Agroindustry Technology Research System,China(BAIC07-2020).

摘　　要：The olfactory system of insects is crucial in modulating behaviors such as host seeking,mating,and oviposition.Odorantbinding proteins(OBPs)are involved in semiochemical recognition.OBPs recognize and bind odorants and transport them to odorant receptors located in olfactory neurons.Harmonia axyridis(Coleoptera:Coccinellidae)is a widely used predacious biological control agent for many agricultural and forestry pests.This study identified 19 OBPs in H.axyridis based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames,including 11‘Classic’OBPs,7‘Minus-C’OBPs and 1‘Plus-C’OBP.They encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to 8.80.Phylogenetic analyses were used to study the relationships between H.axyridis OBPs and OBPs from other species of Coleoptera.Quantitative real-time PCR(qPCR)analysis showed that HaxyOBP2,3,5,8,10,12,13,14,and 15 were highly expressed in antennae of both adult females and males.Moreover,HaxyOBP2,3,5,12,and 15 were more abundantly expressed in antennae than other body parts,while HaxyOBP13 and HaxyOBP14 were expressed predominantly,and at similar levels,in the head and antennae.The other OBP genes were highly expressed in non-olfactory tissues including the thorax,abdomen,legs,and wings.These results provide valuable information for further study of H.axyridis olfaction,which may ultimately enhance its use as a biocontrol agent.

关 键 词：odorant-binding proteins Harmonia axyridis expression profile phylogenetic tree 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]