EGFL7基因对人宫颈癌细胞Hela迁移与侵袭能力的影响  

Effect of EGFL7 gene on migration and invasion of human cervical cell carcinoma cell line Hela

作　　者：许丽娜[1] 李芳[1] 郭东琳[2] 王素琴[1] Xu Lina;Li Fang;Guo Donglin;Wang Suqin(Gynecology Ward 1,Shanxi Provincial People’s Hospital,Taiyuan 030012,China;Clinical Laboratory,Shanxi Provincial People’s Hospital,Taiyuan 030012,China)

机构地区：[1]山西省人民医院妇科一病区,太原030012 [2]山西省人民医院检验科,太原030012

出　　处：《中华内分泌外科杂志》2021年第3期259-263,共5页Chinese Journal of Endocrine Surgery

基　　金：山西省卫生健康委科研课题(2019024)。

摘　　要：目的探究人表皮生长因子样结构域蛋白7(epidermal growth factor-like domain protein 7,EGFL7)基因在人宫颈癌细胞Hela中低表达对其迁移与侵袭能力的影响。方法将人宫颈癌细胞Hela分为实验组(Hela细胞转染EGFL7-siRNA)和对照组(Hela细胞转染Mock-siRNA),采用实时定量PCR法检测两组细胞中EGFL7 mRNA的含量;蛋白免疫印迹实验检测两组细胞EGFL7蛋白的表达量;利用细胞划痕愈合实验和transwell实验检测两组Hela细胞的迁移与侵袭能力。结果siRNA降低了人宫颈癌细胞Hela中EGFL7的蛋白表达;对照组Hela细胞划痕愈百分率为74.1%±6.8%,EGFL7表达降低后宫颈癌细胞划痕愈合百分率为42.0%±4.9%,划痕愈合能力明显下降(P<0.05);Transwell细胞转移结果显示,对照组Hela细胞成功转移的细胞数量为179.24±20.01,而低表达EGFL7的Hela细胞成功转移的细胞数量为79.22±13.16,转染EGFL7-siRNA的细胞转移能力显著下降(P<0.05);侵袭实验结果表明,对照组成功转移的细胞数量为79.35±8.04,EGFL7-siRNA组成功转移的细胞数量为26.98±6.24,低表达EGFL7的Hela细胞侵袭能力明显下降(P<0.05);低表达EGFL7的Hela细胞E-cad表达上调,MMP2/9蛋白的表达下调(均P<0.05)。结论EGFL7低表达会通过上皮间质转化(epithelial to mesenchymal transition,EMT)EMT降低人宫颈癌细胞Hela的迁移与侵袭能力。Objective To investigate the effect of low expression of human epidermal growth factor-like domain protein 7(EGFL7)gene in cervical cancer cell Hela on its migration and invasion ability.Methods Cells in the experimental group used small interfering RNA(siRNA)to target the human EGFL7 gene to reduce the expression of EGFL7 in human cervical cancer cells Hela,and cells in the control group were transfected with Mock-siRNA.Real-time quantitative PCR was used to detect the EGFL7 mRNA content of cancer cells in each group;Western blot was used to detect EGFL7 protein expression of cancer cells in each group;The cell scratch healing experiment and Transwell experiment were used to detect the migration and invasion ability of Hela cells in each group.Results siRNA reduced the protein expression of EGFL7 in human cervical cancer cell Hela.The wound closure percentage of Hela cells in the control group was 74.1%±6.8%.After the expression of EGFL7 was reduced,the percentage of cervical cancer cells was 42%±4.9%,and the wound closure ability was significantly reduced(P<0.05).The results of Transwell cell transfer showed that the number of cells successfully transferred by Hela cells in the control group was 179.24±20.01,while the number of cells successfully transferred by Hela cells with low EGFL7 expression was 79.22±13.16.The transfer ability of cells transfected with EGFL7-siRNA was significantly reduced(P<0.05).The results of invasion experiments showed that the number of successfully transferred cells in the control group was 79.35±8.04,the number of cells successfully transferred in the EGFL7-siRNA group was 26.98±6.24,and the invasion ability of Hela cells with low expression of EGFL7 decreased(P<0.05).The expression of E-cad in Hela cells with low expression of EGFL7 was up-regulated,and the expression of MMP2/9 protein was down-regulated(all P<0.05).Conclusion The low expression of EGFL7 can reduce the migration and invasion ability of cervical cancer cell Hela through the EMT pathway.

关 键 词：EGFL7 宫颈癌 细胞转移能力 

分 类 号：R711.74[医药卫生—妇产科学]