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作 者:王松[1] 吴耀华[2] 李波[1] 敖亚洲[1] 陈泳[1] Wang Song;Wu Yaohua;Li Bo;Ao Yazhou;Chen Yong(Department of Thyroid Surgery,Affiliated Hospital of Chengde Medical University,Chengde 067000,China;Department of Thyroid Surgery,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China)
机构地区:[1]河北省承德医学院附属医院甲状腺外科,承德067000 [2]哈尔滨医科大学附属第一医院甲状腺外科,150000
出 处:《中华内分泌外科杂志》2021年第3期288-292,共5页Chinese Journal of Endocrine Surgery
基 金:承德市科学技术研究与发展计划项目(201801A024)。
摘 要:目的本文旨在探讨外泌体源性LncRNA SPINT1-AS1在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)诊断和预后判断中的价值。方法采用qRT-PCR检测SPINT1-AS1在PTC组织、细胞系(TPC-1、BCPAP、K1和IHH4)和患者血清外泌体及癌旁正常组织、人甲状腺正常细胞系Nthy-ori 3-1和健康志愿者血清外泌体中的表达。CCK-8和克隆形成实验分别检测SPINT1-AS1对PTC细胞增殖和集落形成的影响。双荧光素酶报告实验验证SPINT1-AS1和miR-128-3p、miR-128-3p和ITGA3间的互作用关系。结果SPINT1-AS1在PTC组织和细胞系中的表达较癌旁正常组织和Nthy-ori 3-1细胞系显著升高。敲减SPINT1-AS1能抑制PTC细胞增殖和集落形成。生物信息学分析表明,PTC中存在一个SPINT1-AS1/miR-128-3p/IT-GA3互作用调节网络。双荧光素酶报告实验验证了SPINT1-AS1、miR-128-3p和ITGA3的直接相互作用。此外,还证实了SPINT1-AS1在PTC患者的血清外泌体中显著上调。结论SPINT1-AS1调控miR-128-3p/ITGA3轴促进PTC细胞增殖和克隆形成。外泌体源性SPINT1-AS1可能是PTC诊断和治疗的一个有效分子靶点。Objective To investigate the value of exocrine LncRNA SPINT1-AS1 in diagnosis and prognosis of papillary thyroid carcinoma(PTC).Methods qRT-PCR was used to detect the expression of SPINT1-AS1 in PTC tissues,cell lines(TPC-1,BCPAP,K1 and IHH4),serum exocrine bodies of patients and normal tissues adjacent to cancer,human thyroid normal cell line Nthy-ori 3-1 and healthy volunteers.CCK-8 and colony formation assay were used to detect the effects of SPINT1-AS1 on the proliferation and colony formation of PTC cells.The interaction between SPINT1-AS1 and miR-128-3p,miR-128-3p and ITGA3 was verified by double luciferase report test.Results The expression of SPINT1-AS1 in PTC tissues and cell lines was significantly higher than that in adjacent normal tissues and Nthy-ori 3-1 cell lines.Knockdown of SPINT1-AS1 inhibited the proliferation and colony formation of PTC cells.Bioinformatics analysis showed that there was a SPINT1-AS1/miR-128-3p/ITGA3 interaction regulatory network in PTC.The direct interaction of SPINT1-AS1,miR-128-3p and ITGA3 was verified by double luciferase reporter test.In addition,SPINT1-AS1 was significantly up-regulated in serum exocrine bodies of patients with PTC.Conclusion Our study shows that SPINT1-AS1 regulates the miR-128-3p/ITGA3 axis to promote the proliferation and colony formation of PTC cells.Exocrine SPINT1-AS1 may be an effective molecular target for PTC in diagnosis and treatment of PTC.
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